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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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外源性mtκα基因抑制肝癌细胞hepg2的生长
, PP. 1133-1136
Keywords: 基因,抑制,肿瘤,肝细胞癌,腺病毒载体
Abstract:
目的观察核转录因子的抑制基因mtκα在肝癌细胞株hepg2的表达及对该细胞株生长的影响。方法将由293包装细胞产生的含mtκα的ad腺病毒上清(ad-miκbα)和含包装载体的腺病毒上清(adv)感染hepg2细胞,观察感染后hepg2细胞的绿色荧光蛋白(gfp)表达和miκbα蛋白表达,并观察细胞的平板集落形成数、软琼脂集落形成能力、细胞生长曲线、裸鼠成瘤实验。结果肝癌细胞感染腺病毒后gfp表达阳性,感染含miκbαad腺病毒的肝癌细胞有miκbα蛋白表达;hepg2/ad-miκbα细胞形成的集落数明显比hepg2/adv和hepg2细胞形成的集落数少(p<0.05);hepg2细胞、hepg2/adv细胞在软琼脂内存活,并形成桑椹样细胞集落,而hepg2/ad-miκbα细胞在软琼脂上无生长;细胞生长曲线显示hepg2/ad-miκbα细胞生长速度较hepg2/adv、hepg2亲本细胞有所减慢,但饱和密度无显著性差异。裸鼠接种4周后hepg2/ad-miκbα细胞成瘤率为80%,hepg2/adv细胞成瘤率为100%,肿瘤形成率无显著性差异,hepg2/ad-miκbα细胞形成的肿瘤的体积比hepg2/adv细胞形成的肿瘤的体积明显减小(p<0.05)。结论mtκα基因可以抑制hepg2肝癌细胞的生长。
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