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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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体外三维抗血管生成药物筛选模型的建立
, PP. 1167-1170
Keywords: 内皮细胞,血管生成,模型,药物筛选
Abstract:
目的建立一种新的用于筛选具有抗血管生成作用的体外血管生成模型。方法人脐静脉的内皮细胞(huvecs)在三维胶原纤维系统中培养,制备成具有毛细血管样网状结构模型;3种酪氨酸酶抑制剂genistein,tyrphostina23和lavendustinc被分别加入到模型中,观察它们对血管形成的影响。结果在不加入任何生长因子的条件下,huvecs能迅速重组形成毛细血管样网状结构;3种酪氨酸酶抑制剂均对血管形成有抑制作用。结论该模型具有操作简单、可重复性好、易量化的特点,是一种在体外筛选血管生成抑制剂的理想模型。
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