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OALib Journal期刊
ISSN: 2333-9721
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新生小牛血清对人胎脑海马组织神经干细胞分化的影响
Keywords: 神经干细胞,新生小牛血清,胚胎,海马
Abstract:
目的探讨新生小牛血清对人胎脑海马组织神经干细胞分化的影响。方法采用细胞培养和免疫细胞组化技术,观察不同浓度新生小牛血清对人胎脑海马组织神经干细胞分化的影响。结果新生小牛血清能诱导人神经干细胞分化为神经细胞及神经胶质细胞,在无血清培养基与含不同浓度新生小牛血清培养基中培养的神经干细胞分化为神经细胞和神经胶质细胞的数目在发生变化,并且随着新生小牛血清浓度的增加,神经干细胞分化为神经细胞的数量在减少,分化为神经胶质细胞的数量增加。结论新生小牛血清影响人神经干细胞分化为神经细胞和神经胶质细胞的比例,并与新生小牛血清的浓度有一定的关系。
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