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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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广州地区扎幌样病毒的检出及基因型分析
Keywords: 扎幌样病毒,人类杯状病毒,基因型,反转录聚合酶链反应
Abstract:
目的了解广州腹泻儿童是否存在扎幌样病毒(sapporo-likevirus,slv)感染。方法在2003年秋冬季腹泻流行期间在南方医院儿科收集临床诊断为病毒性腹泻患儿的粪便标本,并采用半套式反转录聚合酶链反应(rt-pcr)方法检测。纯化阳性标本pcr产物并测序,然后将其同genbank中的参比毒株序列比较。结果收集的169份标本中有1份slv(ch03354)pcr扩增阳性,检出率为0.59%,同源性分析表明该毒株属于slvgⅰ-1群。结论广州地区存在slv所致感染,本次实验检出毒株基因型与安徽毒株不同,说明我国不同地区可能存在不同的slv基因型。
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