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OALib Journal期刊
ISSN: 2333-9721
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海马注射淀粉样蛋白β大鼠与正常大鼠脑蛋白质组双向电泳图谱比较
, PP. 553-555
Keywords: 阿尔茨海默病,老年性痴呆,淀粉样蛋白β,双向电泳,蛋白质组
Abstract:
目的比较海马注射淀粉样蛋白β(aβ)大鼠与正常大鼠脑蛋白质组双向电泳(2-de)图谱差异,从蛋白质水平初步探索阿尔茨海默病(ad)发病机制。方法以固相ph梯度等电聚焦为第1向,sds-page垂直电泳为第2向进行2-de。以图像分析软件imagemaster2d-elite分析电泳图谱。结果海马注射aβ大鼠与正常大鼠脑组织2-de图谱分别检出496和491个蛋白点。对2张电泳图进行匹配后,发现有11个蛋白点仅在海马注射aβ大鼠脑蛋白2-de图谱中表达,而有6个蛋白点只在正常对照大鼠检测到。部分蛋白在2组大鼠脑组织中含量发生了明显变化。结论初步建立了ad动物模型比较蛋白质组学的技术方法;差异点的发现为深入理解ad发病机制及研发新药提供了有益的线索。
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