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OALib Journal期刊
ISSN: 2333-9721
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na+通道阻滞极化停搏液在离体大鼠心脏保存中的保护作用
Keywords: 极化停搏液,na+通道阻滞剂,河豚毒素,心肌保护
Abstract:
目的研究含na+通道阻滞剂河豚毒素(ttx)极化停搏液在离体大鼠心脏保存中的心肌保护作用。方法20只wistar大鼠随机均分入st.thomas去极化停搏组(对照组,ⅰ组)和ttx极化停搏组(ⅱ组)。建立离体心脏灌注模型,灌注10min后,分别用st.thomas和ttx极化停搏液2ml经主动脉插管注入,使心脏停搏。随后将鼠心置于相应配伍的停搏液中,10℃保存7h后重新灌注心脏30min。观察两组心脏保存前后在左心室收缩末压力和压力变化速率恢复率、心肌酶漏出、atp含量和心肌超微结构等方面的改变。结果再灌注后,ttx极化停搏组血流动力学各项指标恢复率明显高于ⅰ组(p<0.01),肌酸磷酸激酶和乳酸脱氢酶漏出量低于ⅰ组(p<0.05),atp含量维持在较高水平(p<0.01),心肌超微结构得到较好保护。结论在离体大鼠心脏低温保存过程中ttx极化停搏液心肌保护作用优于sth液。
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