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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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宫颈癌组织中hpv16e7序列多态性分析
, PP. 1272-1275
Keywords: 乳头状瘤病毒,人,e7基因,多态现象,宫颈肿瘤,聚合酶链反应
Abstract:
目的探讨广东地区宫颈癌组织中hpv16肿瘤相关性抗原e7基因序列的多态性。方法采用通用引物pcr直接测序法对宫颈癌标本中的hpv分型,从含有hpv16型的标本中采用自行设计的多重引物通过巢式pcr扩增出hpv16e7,经dna序列测定法检测其基因变异,进而寻找其热点突变。结果50例宫颈癌组织hpv-dna的检出率为78%,其中hpv16和hpv18型混合感染18例,单纯hpv16型感染15例。33例含有hpv16型的标本中扩增出25例hpv16e7,其中17例647位核苷酸“t”变异“c”,导致相应的蛋白质由天冬氨酸变异为丝氨酸。结论广东地区宫颈癌组织中hpv16e7dna序列发生碱基替换的区域主要在647位至846位,热点突变点为nt647和nt846。
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