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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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晚期氧化蛋白产物通过活性氧诱导单核细胞分泌肿瘤坏死因子
, PP. 493-497
Keywords: 晚期氧化蛋白产物,单核细胞,肿瘤坏死因子,nadph氧化酶,p38
Abstract:
目的探讨晚期氧化蛋白产物(aopp)对单核细胞分泌肿瘤坏死因子(tnfα)的影响及可能机制.方法以thp-1和人外周血单核细胞为单核细胞模型,与用次氯酸氧化牛血清白蛋白(bsa)制备的aopp-bsa共同培养,用elisa法检测培养上清tnfα的释放量,用victorwallac1420多标记分析系统检测细胞氧化2,7-二氢二氯荧光素产生的荧光量反映活性氧的产生量:用抗氧化剂吡咯烷二硫代氨基甲酸盐(pdtc),nadph氧化酶抑制剂apocynin和p38磷酸化抑制剂sb20380预处理细胞,探讨aopp诱导单核细胞分泌tnfα的可能机制.结果aopp-bsa能诱导单核细胞tnfα的分泌和细胞内活性氧(ros)的产生.用pdtc预处理细胞,可以清除aopp-bsa诱导产生的ros,同时抑制tnfα的分泌.用apocynin抑制nadph氧化酶及用sb20380抑制p38磷酸化均能有效地抑制aopp-bsa诱导的tnfα分泌.结论aopp可能通过激活单核细胞nadph氧化酶,释放ros,使p38磷酸化,导致tnfα的分泌.
| [1] | witko-sarsatv,friedlanderm,capeillere-blandinc,etal.advancedoxidationproteinproductsasanovelmarkerofoxidativestressinuremia[j].kidneyint,1996,49:1304-13.
|
| [2] | descamps-latschab,witko-sarsatv.oxidativestressinchronicrenalfailureandhemodialysis[j].nephrologie,2003,24:377-9.
|
| [3] | witko-sarsatv,friedlanderm,nguyer-khoat,etal.advancedoxidationproteinproductsasnovelmediatorsofinflammationandmonocyteactivationinchronicrenalfailure[j].jimmunol,1998,161(5):2524-32.
|
| [4] | witko-sarsatv,gaussonv,descamps-latschab.areadvancedoxidationproteinproductspotentialuremictoxins[j]?kidneyintsuppl,2003,84:s11-4.
|
| [5] | yuoa,kitagawas,motoyoshik,etal.rapidprimingofhumanmonocytesbyhumanhematopoieticgrowthfactors[j].blood,1992,79:1553-7.
|
| [6] | carterwo,narayananpk,robinsonjp.intracellularhydrogenperoxideandsuperoxideaniondetectioninendothelialcells[j].jleukocbiol,1994,55(2):253-8.
|
| [7] | zimmermanj,herrlingers,pruya,etal.inflammationenhancescardiovascularriskandmortalityinhemodialysispatients[j].kidneyint,1999,55:648-58.
|
| [8] | albertaw.lipidhydroperoxidegeneration,turnover,andeffectoractioninbiologicalsystems[j].jlipidres,1998,39(8):1529-42.
|
| [9] | panichiv,migliorm,depietros,etal.c-reactiveproteinasamarkerofchronicinflammationinuremicpatients[j].bloodpurif,2000,18(3):183-90.
|
| [10] | stenvinkelp,heimburgero,paultref,etal.strongassociationbetweenmalnutrition,inflammation,andatherosclerosisinchronicrenalfailure[j].kidneyint,1999,55(5):1899-911.
|
| [11] | 梁敏,王力,侯凡凡,等单核细胞活化与终末期肾病炎症反应状态的关系[j].第一军医大学学报,2003,23(8):781-4.liangm,wangl,houff,etal.inflammatorystaresinpatientswithend-stagerenaldisease:roleofmonocyteactivation[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(8):781-4.
|
| [12] | 陈瑗,周攻,侯凡凡,等.氧化应激与慢性肾功能衰竭透析患者动脉粥样硬化的发生[j].中华肾脏病杂志,2002,18(5):377-8.cheny,zhoum,houff,etal.oxidativestressandatherogenesisinhemodialysispatientswithchronicrenalfailure[j].chinjnephrol,2002,18(5):377-8.
|
| [13] | schomigm,eisenhardta,ritze.themicroinflammatorystateofuremia[j].bloodpurif,2000,18(4):327-32.
|
| [14] | kalantar-zadehk,stenvinkelp,pillonl,etal.inflammationandnutritioninrenalinsufficiency[j].advrenreplacether,2003,10(3):155-69.
|
| [15] | 侯凡凡,张训.慢性肾功能衰竭时晚期糖基化终产物的病理生理[j].中华肾脏病杂志,1997,13(5):312-4.houff,zhangx.pathophysiologicalroleofadvancedglycationendproductsinpatientswithchronicrenalfailure[j].chinjnephrol,1997,13(5):312-4.
|
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