|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
睾酮对血管平滑肌细胞中雄激素受体mrna表达的调控
, PP. 298-300
Keywords: 雄激素类,睾酮,受体,雄激素,平滑肌,血管
Abstract:
目的观察睾酮对雄性大鼠血管平滑肌细胞中雄激素受体mrna表达的自身调控作用。方法贴块法培养雄性sd大鼠胸主动脉血管平滑肌细胞(vsmcs),northern印迹分析法检测细胞中雄激素受体mrna水平;采用细胞计数和[3h]-胸腺嘧啶掺入法观察调控过程中细胞数量和dna合成变化情况。结果0~4?mol/l睾酮与静止的vsmcs作用24h,细胞内armrna表达水平呈剂量相关性增加;生理水平睾酮(40nmol/l)与静止的vsmcs作用不同时间(0~24h),细胞内armrna表达水平在24h时方有显著增加;实验过程中细胞数量及dna合成速率均无明显改变。结论血管平滑肌细胞中存在睾酮对雄激素受体mrna表达的自身上调作用,调控过程中细胞活力保持稳定。提示vsmcs中睾酮对ar表达的调控作用部分需要转录水平的参与。
| [1] | higashiurak,mathurrs,halushikapv,etal.gender-relateddifferencesinandrogenregulationofthromboxanea2receptorsinratsmooth-musclecells[j].jcardiolpharm,1997,29(3):311-5.
|
| [2] | lings,daia,williamsmr,etal.testosterone(t)enhancesapoptosis-relateddamageinhumanvascularendothelialcells[j].endocrinology,2002,143(3):1119-25.
|
| [3] | alexandersenp,haarboj,byrjalseni,etal.naturalandrogensinhibitmaleatherosclerosis:astudyincastrated,cholesterol-fedrabbits[j].circres,1999,84(7):813-9.
|
| [4] | bruckb,brehmeu,gugeln,etal.gender-specificdifferencesintheeffectsoftestosteroneandestrogenonthedevelopmentofatherosclerosisinrabbits[j].arteriosclerthrombvascbiol,1997,17(10):2192-9.
|
| [5] | choutm,sudhirk,hutchisonsj,etal.testosteroneinducesdilationofcaninecoronaryconductanceandresistancearteriesinvivo[j].circulation,1996,94(10):2614-9.
|
| [6] | fujimotor,morimotoi,moritae,etal.androgenreceptors,5alpha-reductaseactivityandandrogen-dependentproliferationofvascularsmoothmusclecells[j].jsteroidbiochemmolbiol,1994,50(3-4):169-74.
|
| [7] | nakaoj,changewc,murotasi,etal.testosteroneinhibitsprostacyclinproductionbyrataorticsmoothmusclecellsinculture[j].atherosclerosis,1981,39(2):203-9.
|
| [8] | mizokamia,saigah,matsuit,etal.regulationofandrogenreceptorbyandrogenandepidermalgrowthfactorinahumanprostaticcancercellline,lncap[j].endocrinoljpn,1992,39(3):235-43.
|
| [9] | 马瑞,吴赛珠,吕红松,等.睾酮对大鼠血管平滑肌细胞增殖的影响及其与雄激素受体水平变化的关系[j].中华心血管病杂志,2003,31(6):440-3.mar,wusz,luhs,etal.theeffectoftestosteroneontheproliferationofvascularsmoothmusclecellsanditsrelationtoandrogenreceptorexpressioninthosecells[j].chinjcardiol,2003,31(6):440-3.
|
| [10] | rossr.atherosclerosis:aproblemofthebiologyofarterialwallcellsandtheirinteractionswithbloodcomponents[j].arteriosclerosis,1981,1(5):293-311.
|
| [11] | martinezed,danielsenm.lossofandrogenreceptortranscriptionalactivityattheg(1)/stransition[j].jbiolchem,2002,277(33):29719-29.
|
| [12] | hankeh,lenzc,hessb,etal.effectoftestosteroneonplaquedevelopmentandandrogenreceptorexpressioninthearterialvesselwall[j].circulation,2001,103(10):1382-5.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
