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OALib Journal期刊
ISSN: 2333-9721
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重组腺相关病毒载体介导的绿色荧光蛋白在急性髓性白血病患者骨髓间充质干细胞中的表达
, PP. 281-284
Keywords: 重组腺相关病毒,骨髓间充干细胞,基因治疗
Abstract:
目的探讨重组腺相关病毒载体(raav-2-egfp)是否可高效转导入骨髓间充质干细胞(bmscs)。方法从初发的急性髓性白血病(aml)患者骨髓中分离培养出bmscs,在不同的感染复数(moi=102,103,104,105,106,107)下用包含增强型绿色荧光蛋白(egfp)的raav-2-egfp感染bmscs,寻找最佳的感染条件,并在转染后的不同时间点用倒置荧光显微镜以及流式细胞仪观察其表达情况。结果转染后10~14d,egfp在bmscs中表达,转染效率为0.3%~2%,增加moi亦不能明显增加转染效率。在moi=105的条件下转染后观察了61d,egfp保持低水平长期稳定表达,在转染后的12~33d,egfp阳性的bmscs从起始时的1.16%下降到(0.5~0.6)%,33~61d一直维持在这一水平。结论raav-2-egfp和bmscs可用于体外基因治疗,但极低的转染效率可能是其进一步应用的障碍。
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