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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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阻断磷脂酶c-γ1信号通路对大肠癌细胞凋亡的影响
, PP. 177-180
Keywords: 磷脂酶c-γ1,信号通路,大肠肿瘤,凋亡
Abstract:
目的探讨磷脂酶c-γ1(plc-γ1)信号通路被阻断对大肠癌细胞凋亡状态的影响。方法利用u73122(1、5、10μmol/l)处理细胞以阻断plc-γ1信号通路,光镜、电镜观察细胞形态改变,mtt法评估细胞杀伤效应,流式细胞仪分析凋亡细胞比例。结果plc-γ1信号通路阻断后,大肠癌细胞出现了典型的凋亡形态学改变,存活细胞明显减少,流式细胞仪检测出现典型的凋亡亚二倍体峰,凋亡细胞所占比例达到半数以上。结论阻断plc-γ1信号通路能够启动大肠癌细胞凋亡。
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