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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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大鼠卵巢发育过程中smad4的表达
, PP. 127-131
Keywords: 卵巢,sd大鼠,smad4,免疫组化,rt-pcr
Abstract:
目的了解转化生长因子-β超家族(tgf-βs)在调节卵泡发育过程中的信号转导模式,以探讨在不同发育阶段大鼠卵巢中smad4蛋白及mrna的表达。方法选择不同发育时期大鼠卵巢,运用免疫组化方法检测卵巢中smad4蛋白表达,并进行图像分析;采用半定量逆转录聚合酶链反应(rt-pcr)方法检测smad4mrna在卵巢中的表达,以gapdh作为内对照与特异性smad4同时进行扩增,并计算smad4和gapdh的rt-pcr产物积分吸光度比值来表示smad4mrna的含量。结果smad4广泛表达于卵巢组织,但主要表达在卵泡中,其表达部位和强度随卵巢的成熟度不同而变化;在卵巢发育早期,smad4主要在原始卵泡和窦前卵泡中表达,而在间质中的表达较弱;随着卵巢的发育成熟,smad4在间质中的表达逐渐增强,性成熟后,在窦状及成熟卵泡颗粒细胞和卵泡膜的表达与间质细胞比较无显著差异(p>0.05)。smad4在卵泡中的表达强度也发生了变化,即随着卵泡的发育,smad4在窦状及成熟卵泡卵母细胞的表达明显减弱,与窦前卵泡卵母细胞比较有显著差异(p<0.05,p<0.01);与窦前卵泡比较,smad4在卵泡膜细胞的表达逐渐增强(p<0.01),而在各级卵泡颗粒细胞中的表达无显著差异(p>0.05)。rt-pcr结果显示各阶段卵巢均有mrna的表达,从第3周起smad4mrna的表达明显增强,smad4和gapdh积分吸光度比值与出生后1d的差异均有统计学意义(p<0.05)。结论卵巢内存在smad4,提示tgf-β家族对卵泡发育的调节是通过smad信号转导模式实现的。
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