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OALib Journal期刊
ISSN: 2333-9721
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pcr直接测序法检测宫颈癌组织中人乳头瘤病毒dna
, PP. 201-203
Keywords: 人类乳头瘤病毒,聚合酶链反应,测序分析,宫颈癌
Abstract:
目的探索检测宫颈癌组织中人乳头瘤病毒(hpv)dna的有效手段。方法采用hpv通用引物对宫颈癌标本中的hpvl1区基因序列进行pcr扩增,根据pcr产物序列分析对hpv分型。结果50例宫颈癌组织hpvdna检出率为78%,其中hpv16和hpv18型混合感染最多见。检测出1例hpv58型,其l1区基因序列与德国标准株58型比对,未发现碱基变异。结论pcr直接测序法是对宫颈癌组织中hpv检测和分型的一种有效方法,并可检测到hpv少见的特殊类型,同时也能提供已知的hpv型别的突变信息。
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