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OALib Journal期刊
ISSN: 2333-9721
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x射线照射后鼻咽癌细胞多药耐药基因的表达
, PP. 646-649
Keywords: 鼻咽肿瘤,肿瘤细胞,培养的,药物耐受性,x线,p-糖蛋白类
Abstract:
目的通过检测射线照射前后鼻咽癌cne1细胞多药耐药基因(mdr1基因)及其编码产物p糖蛋白(p-gp)的表达和功能为临床鼻咽癌放化疗顺序提供参考。方法利用rt-pcr、westernblotting和流式细胞仪检测射线照射前后cne1细胞的mdr1基因和p-gp的表达及对柔红霉素的外排功能。结果鼻咽癌cne1细胞射线照射前mdr1基因、p-gp不表达;照射后较长时间内mdr1基因、p-gp均明显表达;对柔红霉素的摄取较射线照射前低。结论鼻咽癌cne1细胞射线照射后化疗敏感性降低,提示临床对于中晚期鼻咽癌的治疗应考虑先化疗再放疗即诱导化疗的治疗方案。
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