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OALib Journal期刊
ISSN: 2333-9721
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肿瘤坏死因子对滋养细胞侵袭性的影响
Keywords: 滋养细胞,侵袭,肿瘤坏死因子,基质金属蛋白酶,组织抑制因子
Abstract:
目的探讨外源性肿瘤坏死因子(tnf-α)及其多克隆抗体对原代滋养细胞侵袭力的影响。方法采用逆转录多聚酶链反应(rt-pcr)方法,检测tnf-α、antitnf-α、tnf-α+antitnf-α对原代滋养细胞基质金属蛋白酶(mmp-2)及其组织抑制因子(timp-2)表达的影响。结果原代滋养细胞表达mmp-2,经过5ng/ml的tnf-α、tnf-α+antitnf-α分别刺激培养原代滋养细胞48h后,细胞mmp-2的表达显著性提高(p<0.001),50ng/ml的antitnf-α刺激培养后mmp-2的表达显著性降低(p<0.001);各组间mmp-2的表达存在显著性差异(p<0.001),但是均未见明显的timp-2的表达。结论外源性细胞因子tnf-α可以增强滋养细胞的侵袭力,多克隆抗体antitnf-α则降低其侵袭力。
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