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OALib Journal期刊
ISSN: 2333-9721
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nk/lak细胞对人口腔癌耐细胞药株杀伤活性的观察
, PP. 392-396
Keywords: 免疫,细胞,药物耐受性,肿瘤细胞,培养的,杀伤细胞,淋巴因子激活,杀伤细胞,天然
Abstract:
目的明确免疫活性细胞对肿瘤细胞的杀伤活性是否与肿瘤的耐药性相关。方法采用连续形态学观察及mtt比色法,研究淋巴因子激活的杀伤(lak)细胞及自然杀伤(nk)细胞,对人口腔癌耐药细胞株kbv200耐药性逆转前后及亲本敏感株kb的杀伤活性。(1)在肿瘤细胞与lak细胞共育后3h内连续在倒置显微镜下观察lak细胞对上述3者的杀瘤效应;(2)采用mtt比色法检测nk及lak对3株细胞的杀伤率。结果与kb细胞组相比,在kbv200和kbv200+维拉帕米组,lak细胞出现在靶细胞周围的时间早、数量多,伸出伪足的lak细胞比率高,出现集落样细胞团块时间亦早。nk、lak细胞对kbv200细胞株耐药性逆转前后的杀伤率均明显高于敏感株kb(p<0.05),而对耐药株耐药性逆转前后的杀伤率无统计学差异(p>0.05);lak对各株的杀伤活力均明显强于nk细胞(p<0.05)。结论免疫活性细胞对kbv200细胞株有较强的杀伤作用,逆转耐药性不降低免疫活性细胞杀伤活力,提示细胞过继免疫治疗可能成为控制化疗耐药病人病情发展的一个有效手段。
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