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OALib Journal期刊
ISSN: 2333-9721
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己酮可可碱对人肝癌hepg2细胞的放射增敏作用及其作用机制
, PP. 382-385
Keywords: 放射肿瘤学,肝肿瘤/放射疗法,细胞周期/药物作用,放射敏感性/药物作用,己酮可可碱,hepg2细胞系
Abstract:
目的研究己酮可可碱(pentoxifylline,ptx)对人肝癌hepg2细胞的放射增敏作用并初步探讨其作用机制。方法应用mtt法检测ptx药物毒性实验,用克隆形成实验观察ptx对放射敏感性的影响,用流式细胞仪(fcm)技术分析x射线照射对hepg2细胞周期分布和ptx对放射引起的细胞周期阻滞的影响。结果不同浓度的ptx作用于肝癌hepg2细胞24h后,其细胞毒性呈剂量依赖性,最适浓度为2mmol/l。ptx能显著降低放射后hepg2细胞的克隆形成率,其放射增敏比为1.31±0.16。单纯照射组照射可以导致hepg2细胞g2期阻滞,单纯照射组及照射6gy加2mmol/lptx组的细胞20hg2-m期比例分别为32.15%和19.52%,ptx能够去除放射引起的hepg2细胞g2期阻滞。结论ptx对hepg2细胞有放射增敏作用,其机制可能与ptx去除放射引起的g2期阻滞等因素有关。
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