|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
运用组织芯片技术检测cox-2在结直肠癌中的表达及其临床意义
, PP. 1524-1528
Keywords: 组织芯片,cox-2,结直肠癌,预后
Abstract:
目的探讨结直肠癌组织中cox-2的表达及其与各临床病理因素的关系,评价cox-2在结直肠癌预后判断中的价值。方法应用组织芯片技术结合免疫组织化学sabc法,检测126例早中期结直肠癌组织中cox-2的表达情况,回顾性分析cox-2与各临床病理因素及预后之间的关系。结果根据免疫组化染色强度,所有病例被分为cox-2高表达组和低表达组,其中高表达组有32例(25.4%),低表达组有94例(74.6%)。cox-2在结直肠癌中表达情况与年龄、性别、肿瘤大小、肿瘤部位、组织学类型、浸润深度、淋巴是否转移、dukes分期均无相关。然而,高表达的cox-2与肿瘤复发、特别是血行转移显著相关(p<0.05)。两组之间生存率具有显著差异(p=0.0067),cox-2高表达组术后五年生存率显著低于低表达组。多因素回归模型分析结果显示,在潜在的预后因素中(年龄、性别、肿瘤大小、肿瘤部位、组织学类型、淋巴是否转移、dukes分期、cox-2表达),cox-2表达和dukes分期可作为是结直肠癌根治术后独立预后因素,cox-2的检测可作为结直肠癌患者预后判断的一个有价值的指标。结论高表达的cox-2与肿瘤复发、特别是血行转移显著相关,cox-2的检测可作为结直肠癌患者预后判断的一个有价值的指标。应用组织芯片高效检测临床组织样本具有快速、方便、经济、准确的优点。
| [1] | kinoshitat,takahashiy,sakashitat,etal.growthstimulationandinductionofepidermalgrowthfactorreceptorbyoverexpressionofcyclooxygenases1and2inhumancoloncarcinomacells[j].biochimbiophysacta1999,1438(1):120-30.
|
| [2] | hlat,neilsonk.humancyclooxygenase-2cdna[j].procnatlacadsciusa,1992,89(16):7384-8.
|
| [3] | lynchpm.cox-2inhibitioninclinicalcancerprevention[j].oncology(huntingt),2001,15(3supple5):21-6.
|
| [4] | tsujiim,kawanos,tsujis,etal.cyclooxygenaseregulatesangiogenesisinducedbycoloncancercells[j].cell1998,93(5):705-16.
|
| [5] | cianchif,cortesinic,bechip,etal.up-regulationofcyclooxygenase-2geneexpressioncorrelateswithtumorangiogenesisinhumancolorectalcancer[j].gastroenterology,2001,121(6):1339-47.
|
| [6] | danielto,liuh,morrowjd,etal.thromboxanea2isamediatorofcyclooxygenase-2-dependentendothelialmigrationandangiogenesis[j].cancerres,1999,59(18):4574-7.
|
| [7] | wuqm,lisb,wangq,etal.theexpressionofcox-2inesophagealcarcinomaanditsrelationtoclinicopathologiccharacteristic[j].worldjgastroenterol,2001,9(1):11-4.
|
| [8] | tsujiim,kawanos,duboisrn.cyclooxygenase-2expressioninhumancoloncancercellsincreasemetastaticpotential[j].procnatlacadsciusa,1997,94(7):3336-3340.
|
| [9] | yamamotos,konishii,mandaim,etal.expressionofvascularendothelialgrowthfactor(vegf)inepithelialovarianneoplasm:correlationwithchinicopathologyandpatientsurvival,andanalysisofserumvegf[j].brjcancer,1997,76(9):1221-7.
|
| [10] | lig,yangt,yanj.cyclooxygenase-2increasedtheangiogenicandmetastaticpotentialoftumorcells[j].biochembiophysrescommun,2002,299(5):886-90.
|
| [11] | yamauchit,watanabem,kubotat,etal.cyclooxygenase-2expressionasanewmarkerforpatientswithcolorectalcancer[j].discolonrectum,2002,45(1):98-103.
|
| [12] | hullma,fenwicksw,chappleks,etal.cyclooxygenase-2expressionincolorectalcancerlivermetastases[j].clinexpmetastasis,2000,18(1):21-7.
|
| [13] | guptara,duboisrn.colorectalcancerpreventionandtreatmentbyinhibitionofcyclooxygenase-2[j].natrevcancer,2001,1(1):11-21.
|
| [14] | muratah,kawanos,tsujis,etal.cyclooxygenase-2overexpressionenhanceslymphaticinvasionandmetastasisinhumangastriccarcinoma[j].amjgastroenterol,1999,94(2):451-5.
|
| [15] | lig,yangt,yanj.cyclooxygenase-2increasedtheangiogenicandmetastaticpotentialoftumorcells[j].biochembiophysrescommun,2002,299(5):886-90.
|
| [16] | sunb,wuyl,zhangxj,etal.effectsofsulindacongrowthinhibitionandapoptosisinductioninhumangastriccancercells[j].worldjgastroenterol,2001,9(9):997-1002
|
| [17] | wuyl,sunb,zhangxj,etal.growthinhibitionandapoptosisinductionofsulindaconhumangastriccancercells[j].worldjgastroenterol,2001,7(7):796-800.
|
| [18] | tiang,yujp,luohs,etal.effectofnimesulideonproliferationandapoptosisofhumanhepatomasmmc-7721cells[j].worldjgastroenterol,2002,8(3):483-7.
|
| [19] | zhangjz,huangyw,liudm,etal.tissuemicroarraytechnologyanditspreliminaryapplicationinlargeintestinalcarcinoma[j].worldjgastroenterol,2002,10(2):157-60.
|
| [20] | shenzx,caog,sunj.theclinicalandpathologyeffectofcox-2mrnaexpressionincolorectalcancers[j].worldjgastroenterol,2001,9(9):1082-4.
|
| [21] | wolffh,saukkonenk,anttilas,etal.expressionofcyclooxygenase-2inhumanlungcarcinoma[j].cancerres1998,58(22):4997-5001.
|
| [22] | shiotag,okubom,noumit,etal.cyclooxygenase-2expressioninhepatocellularcarcinoma[j].hepatogastroenterology,1999,46(25):407-12
|
| [23] | chenws,weisj,liujm,etal.tumorinvasivenessandlivermetastasisofcoloncancercellscorrelatedwithcyclooxygenase-2expressionandinhibitedbyacox-2-selectiveinhibitor,etodolac[j].intjcancer,2001,91(6):894-9.
|
| [24] | liuxh,kirschenbuama,yaos,etal.up-regulationofvascularendothelialgrowthfactorbycobaltchloride-simulatedhypoxiaismediatedbypersistentinductionofcyclooxygenase-2inametastatichumanprostatecancercelllines[j].clinexpmetastasis1999,17(8):687-94.
|
| [25] | cianchif,cortesinic,bechip,etal.up-regulationofcyclooxygenase2geneexpressioncorrelateswithrumorangiogenesisinhumancolorectalcancer[j].gastroenterology2001,121(6):1339-47.
|
| [26] | muratah,kawanos,tsujis,etal.cyclooxygenase-2overexpressionenhanceslymphaticinvasionandmetastasisinhumangastriccarcinoma[j].amjgastroenterol,1999,94:451-5.
|
| [27] | 曹歌,沈志祥.前列腺素与结直肠肿瘤[j].世界华人消化杂志,2001,9(4):431-4.caog,shenzx.prostaglandinandthecarcinomaofcolorectal[j].worldjgastroenterol,2001,9(4):431-4.
|
| [28] | kosakat,miyataa,iharah,etal.characterizationofthehmangene(ptgs2)encodingprostaglandin-endoperoxidesynthase[j].eurjbiochem,1994,221(3):889-97.
|
| [29] | moritai,schindlerm,regiermk,etal.differentintracellularlocationsforprostaglandinendoperoxidehsynthase-1and-2[j].jbiolchem,1995,270(8):10902-8.
|
| [30] | tomozawas,nagawah,tsunon,etal.inhibitionofhaematogenousmetastasisofcoloncancerinmicebyaselectivecox-2inhibitor,jte-522[j].brjcancer,1999,81(8):1274-79.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
