|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
人cd40分子基因转染人脐静脉内皮细胞ecv-304
, PP. 1474-1477
Keywords: cd40,人脐静脉内皮细胞,转染,基因表达
Abstract:
目的构建人cd40的真核表达载体,建立持续？稳定高表达cd40的人脐静脉内皮细胞ecv-304。方法将含有cd40的克隆载体pucd40酶切后,再克隆到真核表达载体pcdna3.1中,构建重组质粒pcdna3.1(+)/cd40,并对重组质粒进行酶切鉴定。然后用脂质体法将重组质粒转染ecv-304,g418筛选转染的细胞。rt-pcr？western-blotting和流式细胞仪定性定量检测转染后的ecv-304的cd40的表达情况。结果经酶切鉴定,重组体中已插入目的基因片段cd40。rt-pcr和western-blotting证实,重组质粒转染的ecv-304中有cd40基因的表达,流式细胞仪检测转染后的ecv-304的cd40的表达率为95%。结论成功构建了真核表达载体pcdna3.1(+)/cd40,并建立了高表达cd40的ecv-304,为筛选抗动脉粥样硬化药物奠定了基础。
| [1] | schonbecku,libbyp.thecd40/cd154receptor/liganddyad[j].cellmollifesci,2001,58(1):4-43.
|
| [2] | armitagerj,fanslowwc,strockbinel,etal.molecularandbiologicalcharacterizationofamurineligandforcd40[j].nature,1992,357(6373):80-2.
|
| [3] | rossr.atherosclerosis-aninflammatorydisease[j].nengljmed,1999,340(2):115-26.
|
| [4] | machf,schonbecku,sukhovagk,etal.functionalcd40ligandisexpressedonhumanvascularendothelialcells,smoothmusclecells,andmacrophages:implicationsforcd40-cd40ligandsignalinginatherosclerosis[j].procnatlacadsciusa,1997,94(5):1931-6.
|
| [5] | phippsrp,koumasl,leunge,etal.thecd40-cd40ligandsystem:apotentialtherapeutictargetinatherosclerosis[j].curropininvestdrugs,2001,2(6):773-7.
|
| [6] | linr,liujt,ganwj.c-reactiveprotein-inducedexpressionofcd40-cd40landtheeffectoflovastatinandfenofibrateonitinhumanvascularendothelialcells[j].biolpharmbull,2004,27(10):1537-43.
|
| [7] | linr,liujt,pengn.lovastatinreducesnuclearfactorkappabactivationinducedbyc-reactiveproteininhumanvascularendothelialcells[j].biolpharmbull,2005,28(9):1630-4.
|
| [8] | lutgense,daemenmj.cd40-cd40linteractionsinatherosclerosis[j].trendscardiovascmed,2002,12(1):27-32.
|
| [9] | bruemmerd,riggersu,holzmeisterj,etal.expressionofcd40invascularsmoothmusclecellsandmacrophagesisassociatedwithearlydevelopmentofhumanatheroscleroticlesions[j].amjcardiol,2001,87(1):21-7.
|
| [10] | machf,schonbecku,libbyp.cd40signalinginvascularcells:akeyroleinatherosclerosis[j].atherosclerosis,1998,137(suppl):s89-95.
|
| [11] | 魏尔清,沈建中.后基因组时代药理学研究趋势[j].生理科学进展,2002,33(1):7-11.weieq,shenjz.trendsinthepost-genomicpharmacologicalresearch[j].progphysiolsci,2002,33(1):7-11.
|
| [12] | 贺浪冲,耿信笃.细胞膜受体色谱法-研究药物与受体作用的新方法[j].生物医药色谱进展,1996,3:8-9.
|
| [13] | 高琨,贺浪冲,杨广德.用细胞膜色谱法筛选研究红毛七中的有效成分[j].中国药学杂志,2003,38(1):14-6.gaok,helc,yanggd.screeningtheeffectivecomponentofleonticerobustumbycellmembranechromatography[j].chinpharmj,2003,38(1):14-6.
|
| [14] | 张汉利,杨广德,贺浪冲,等.太白花活性成分的筛选与药理作用相关性研究[j].中国药学杂志,2003,38(2):92-4.zhanghl,yanggd,helc,etal.studiesonscreeningtheeffectivecomponentsofcladoniaalpestrisanditscorrelationwithpharmacologicaleffects[j].chinpharmj,2003,38(2):92-4.
|
| [15] | 王锐平,陈蓁,贺浪冲,等.细胞膜色谱法筛选红花中的有效成分[j].陕西中医,2004,25(7):643-4.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
