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OALib Journal期刊
ISSN: 2333-9721
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应用生物信息学技术筛查绒毛膜癌相关基因
, PP. 1-6
Keywords: 绒毛膜癌,葡萄胎,聚类分析,基因表达谱,生物信息学
Abstract:
目的比较和挖掘葡萄胎、绒毛膜癌基因表达谱数据,筛选与绒毛膜癌恶性演进相关的新基因。方法分别以葡萄胎、绒毛膜癌为检测组,均以正常胎盘绒毛为对照组,在对两组各3例葡萄胎、绒毛膜癌进行基因芯片检测的基础上,对其共表达基因表达谱数据进行分层聚类分析,找到有相似表达趋势的候选基因,应用生物信息学方法,进行电子northern分析及基因结构功能初步分析,用半定量rt-pcr技术对3个筛选基因进行初步鉴定。结果在所有3例绒毛膜癌基因表达数据中找到共表达候选基因52条,经聚类分析初步筛选出绒毛膜癌相关基因19条,主要包括dynamin(l07807),zincfingerproteinznf184(u6656),calmodulin(u12022),carboxypeptidasem(bc022276),kataninp60(af056022),calcineurinbindingproteincabin1(nm_012295),transducin-likeenhancerprotein(tle1,m99435)等。结论通过对基因表达谱数据的分析和挖掘,初步筛选了绒毛膜癌相关基因,为寻找绒毛膜癌发生与演进的关键基因提供了新的线索。
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