pseudomonasputidakt2442制备中长链聚羟基烷酸酯
DOI: 10.3969/j.issn.1671-7627.2003.06.006, PP. 25-28
Keywords: 中长链聚羟基烷酸酯,油酸,玉米油水解物,补料分批培养,pseudomonasputidakt2442
Abstract:
初步探讨了利用pseudomonasputidakt2442制备中长链聚羟基烷酸酯(mcl-phas)的工艺,考察了不同碳源对菌体生长和产物合成的影响.在2.5l发酵罐上采用高密度细胞培养制备:以油酸为碳源,细胞干重达120g/l,mcl-phas质量浓度达40g/l,产物质量分数为34%,生产速率为0.965g·l-1·h-1;以玉米油水解物为碳源,细胞干重达105g/l,mcl-phas达28g/l,产物质量分数为26.5%,生产速率为0.667g·l-1·h-1.以玉米油水解物为碳源所得细胞浓度、产物浓度与以油酸为碳源所得结果比较接近,有利于降低生产成本.
| [1] | 王金平.气相色谱法分析棕榈油及其它油脂的脂肪酸组成[j].日用化学工业,1993(6):26-28.
|
| [2] | brauneggg,sonnleitnerb,laffertyrm.arapidgaschromatographicmethodforthedeterminationofpoly-3-hydroxybutyricacidinmicrobialbiomass[j].europeanjournalofappliedmicrobiologyandbiotechnology,1978(1):29-37.doi:10.1007/bf00500854.
|
| [3] | leesy.bacterialpolyhydroxyalkonoates[j].biotechnologyandbioengineering,1996(1):1-14.doi:10.1002/(sici)1097-0290(19960105)49:1<1::aid-bit1>3.3.co;2-1.
|
| [4] | poiriery,nawrathc,somervillec.productionofpolyhydroxyalkanoates,afamilyofbiodegradableplasticsandelastomers,inbacteriaandplants[j].bio/technology,1995(2):142-150.
|
| [5] | leesy,changhn.productionofpoly-(hydroxyalkanoicacid)[j].advancesinbiochemicalengineering/biotechnology,1995(1):27-58.
|
| [6] | 黄显慈.制取优质脂肪酸甲酯的新方法[j].甘肃化工,1993(3):37.
|
Full-Text
