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OALib Journal期刊
ISSN: 2333-9721
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柱前衍生化hplc法测定发酵液中l-瓜氨酸和l-鸟氨酸含量
, PP. 77-80
Keywords: 高效液相色谱,柱前衍生,l瓜氨酸,l鸟氨酸
Abstract:
建立一种应用异硫氰酸苯酯将l瓜氨酸和l鸟氨酸柱前衍生后进行定量分析的高效液相色谱法.实验结果表明，瓜氨酸在02～20mg/ml范围内线性回归显著（r2=09998）,平均回收率9987%；鸟氨酸在02～20mg/ml范围内线性回归显著（r2=09997）,平均回收率10092%.应用该方法对发酵液中l瓜氨酸和l鸟氨酸含量进行测定取得了满意的结果，能有效指导谷氨酸棒杆菌l瓜氨酸的发酵生产.
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