保护氨基酸与羟基树脂的连接反应
DOI: 10.3969/j.issn.1671-7627.2007.01.003, PP. 12-15
Keywords: fmoc-氨基酸,wang树脂,酰氯,活化酯
Abstract:
在多肽固相合成中,分别采用酰氯法和活化酯法,研究了20种fmoc氨基酸与wang树脂的连接反应.除精氨酸外,其他氨基酸与树脂的连接效率均能达到80%以上.酰氯法反应速率快、操作简便,优于活化酯法.在酰氯法中,对于精氨酸,选择弱极法溶剂如dcm,延长反应时间至20h,连接效率接近80%.
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