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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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大肠杆菌k5多糖生物合成条件的优化
DOI: doi:10.3969/j.issn.1671-7627.2012.06.014, PP. 69-73
Keywords: k5多糖,生物合成条件,大肠杆菌
Abstract:
采用plackett-burman实验、正交试验设计方法对k5多糖的生物合成条件进行优化。然后,在摇瓶及5l发酵罐中进行k5多糖发酵过程研究。结果表明:最佳培养基组成为麦芽糖20g/l,蛋白胨15g/l,mncl4·4h2o0.1g/l,mgso4·7h2o2.0g/l,kh2po42.0g/l,k2hpo49.7g/l,脱水枸橼酸钠0.5g/l;250ml摇瓶最佳装液量为15ml。优化后5l发酵罐中k5多糖的质量浓度为1.8g/l,较优化前的0.3g/l提高了5倍。
| [1] | linhardtrj.heparin:structureandactivity[j].jmedchem,2003,46(13):2551-2554.
|
| [2] | zhangzq,mccallumsa,xiej,etal.solutionstructuresofchemoenzymaticallysynthesizedheparinanditsprecursors[j].jamchemsoc,2008,130(39):12998-13007.
|
| [3] | wangz,lym,zhangf,etal.e.colik5fermentationandthepreparationofheparosan,abioengineeredheparinprecursor[j].biotechnolbioeng,2010,107(6):964-973.
|
| [4] | 张书芬,史萍萍,王全林,等.液相色谱示差折光法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖[j].食品科学,2008,29(6):280-283.
|
| [5] | 姜瑞芝,王颖,陈英红,等.猴头菌寡糖的化学研究[j].中国药学杂志,2008,43(5):341-344.
|
| [6] | linhardtrj,toidat.roleofglycosaminoglycansincellularcommunication[j].accchemres,2004,37(7):431-438.
|
| [7] | manzonim,bergomis,rollinim.influenceofthecultureconditionsonextracellularlyaseactivityrelatedk5polysaccharide[j].biotechnollett,2000,22(1):81-85.
|
| [8] | linahlu,lijp,orestep,etal.generationof“neoheparin”frome.colik5capsularpolysaccharide[j].jmedchem,2005,48(2):349-352.
|
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