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OALib Journal期刊
ISSN: 2333-9721
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介孔tio2的孔结构调控及其蛋白质固定化性能
DOI: doi:10.3969/j.issn.1671-7627.2012.06.002, PP. 7-11
Keywords: 介孔tio2,孔径,牛血清蛋白,吸附,固定化
Abstract:
以水合二钛酸(h2ti2o5·xh2o)为前驱体,通过改变热处理条件制备不同孔径尺寸的介孔tio2。采用x线衍射仪、场发射扫描电子显微镜、n2吸附-脱附方法对tio2的结构进行表征,同时以牛血清蛋白(bsa)为模型蛋白,考察介孔tio2的孔结构对bsa固定化性能的影响。结果表明:介孔tio2的孔径可实现9～30nm范围内可调,其中650℃焙烧得到的介孔tio2孔径为21.6nm,对bsa的吸附量达69.6mg/g,优于其他孔径尺寸的介孔tio2,且与纳米tio2(p25)的吸附量相当。吸附的bsa分子进入tio2的孔道内,介孔tio2比p25具有较好的吸附稳定性,经过6次循环后吸附的bsa残余量仍有65%,而p25上bsa的残余量仅为17%。介孔tio2为微米级颗粒,有利于从反应体系中分离回收,是一种极有潜力的生物分子固定化载体。
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