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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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氧化葡萄糖酸杆菌驯化选育及其产二羟丙酮
, PP. 10-14
Keywords: 甘油,氧化葡萄糖酸杆菌,二羟丙酮,驯化,发酵
Abstract:
？通过梯度增加种子培养基中甘油浓度驯化氧化葡萄糖酸杆菌(gluconobateroxydans)，有效地提高了该菌对甘油的耐受性，菌株产二羟丙酮水平比驯化前提高了29%，在此基础上，采用单因素试验，对其发酵工艺进行优化。考察甘油质量浓度、酵母膏质量浓度、caco3质量浓度、接种量对发酵的影响，结果显示最佳工艺条件为：甘油质量浓度为60g/l，酵母膏质量浓度为5g/l，caco3质量浓度为10g/l，接种量4%，在此条件下，经72h摇瓶发酵甘油转化率达96%，二羟丙酮产量可达574g/l，75l发酵罐分批发酵54h时甘油转化率达97%，二羟丙酮产量可达582g/l。？
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