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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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生物转化法重组谷氨酸脱羧酶合成γ-氨基丁酸
, PP. 85-90
Keywords: 谷氨酸脱氢酶(gad),工程菌,酶法合成,c-氨基丁酸(gaba)
Abstract:
成功构建了一个高效表达大肠杆菌谷氨酸脱羧酶gad来源的重组质粒pet28a-gada,并转化e.colibl21(de3),工程菌株经0.4mmol/liptg或1g/l的乳糖,37℃诱导表达8h,粗酶液的酶活达到12μ/ml,大约是出发菌株e.colik-12的60倍.工程菌1.15u粗酶液以31g/ll-谷氨酸钠为底物,37℃、ph4.0条件下反应4h,γ-氨基丁酸的生成量达到19.57g/l,l-谷氨酸钠的转化率为93%,从而为γ-氨基丁酸的生产提供了很好的前景
| [1] | hayakawak,kimuram,kasahak,eta.leffectofc-aminobutyricacidenricheddairyproductonthebloodpressureofspontaneouslyhypertensiveandnormotensivewistar-kyotorats[j].britishjournalofnutrition,2004,92(3):411-417.
|
| [2] | cossartr,bernardc,ben-ariy.multiplefacetsofgabaergicneuronsandsynapses:multiplefatesofgabasignallinginepilepsies[j].trendsinneurosciences,2005,2(28):108-115.
|
| [3] | tadashio,tomokos.effectofdefattedricegermwithgabaforsleeplessness,depression,autonomicdisorderbyoraladministration[j].nipponshokuhinkagakukagakukaish,i2000,47(8):596-603.
|
| [4] | abbjasovale,tuzovdg.methodofsynthesisofc-aminobutyricacid:ru,2202538[p].2003-04-201.
|
| [5] | 赵艳,梁新乐,张虹.大肠杆菌谷氨酸脱羧酶的结构、功能及其基因表达调控[j].食品与发酵工业,2006,32(7):75-78.
|
| [6] | komatsuzakin,shimaj,kawamotos,eta.lproductionofc-aminobutyricacid(gaba)bylactobacillusparacaseiisolatedfromtraditionalfermentedfoods[j].foodmicrobiology,2005,22:497-504.
|
| [7] | 许建军,江波,许时婴.比色法快速测定乳酸菌谷氨酸脱羧酶活力及其应用[j].微生物学通报,2004,31(2):66-71.
|
| [8] | capitanig,debiased,aurizic,eta.lcrystalstructureandfunctionalanalysisofescherichiacoliglutamatedecarboxylase[j].theembojourna,l2003,22(16):4027-4037.
|
| [9] | robertse,frankels.c-aminobutyricacidinbrain:itsformationfromglutamicacid[j].journalofbiologicalchemistry,1950,187:55-631.
|
| [10] | 郭晓娜,朱永义,朱科学.生物体内gaba的研究[j].氨基酸和生物资源,2003,25(2):70-72.
|
| [11] | nomuram,kimotoh,someyay,eta.lproductionofc-aminobutyricacidbycheesestartersduringcheeseripening[j].dairyfoods,1998,81:1486-1491.
|
| [12] | choisi,leejw,parksm,eta.limprovementofgamma-aminobutyricacid(gaba)productionusingcellentrapmentoflactobacillusbrevisgaba057[j].journalofmicrobiologyandbiotechnology,2006,16:562-568.
|
| [13] | 陈海军,林亲录,王婧,等.c-氨基丁酸测定方法的研究[j].食品工业科技,2007,28(5):235-247.
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