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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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萼花臂尾轮虫(brachionuscalyciflorus)非混交雌体与雄体基因组dna的rapd分析
, PP. 109-112
Keywords: 萼花臂尾轮虫,rapd,非混交雌体,雄体,遗传背景
Abstract:
采用rapd分析技术对萼花臂尾轮虫2种不同性别个体的基因组dna进行对比分析,探讨萼花臂尾轮虫非混交雌体和雄体性别分化的遗传背景.所用的30条随机引物中,有10条引物在两者基因组中扩增出多态性片段,其中有二者共有条带,也有二者差异条带,显示了萼花臂尾轮虫非混交雌体和雄体遗传背景上的差异.其中引物b15和b18扩增条带具有良好的稳定性和重复性,进一步选取了用此二引物扩增出的雌雄特异性片段各一条进行回收、克隆和测序,测序结果已提交到genbank数据库,序列号分别为:ay907696和ay898611,同源比较未发现有高同源性的已知序列与之匹配,表明它们可能是2条新的dna序列.
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