OALib Journal期刊
雌激素对小鼠肠道甜味传导相关蛋白表达的影响
李国梁 ,
陈梦玲 ,
郑 ,
敏 ,
王 ,
兵 ,
耿倩倩 ,
张羽歆 ,
梁 ,
沛 ,
詹月华 ,
张根华 Keywords: 卵巢切除术 , 雌激素 , 肠道 , C57BL/6小鼠ovariectomy , estrogen , intestines , C57BL/6 mice
Abstract:
雌激素与甜味感知紧密相连,可以通过影响机体对甜味的感知能力而改变人们对碳水化合物的摄取,但雌激素在肠道味觉中所起的作用尚不明确. 本实验以行卵巢切除术为手段构建雌激素缺乏小鼠模型,采用实时荧光定量PCR和蛋白免疫印迹技术,从分子和蛋白质水平检测雌激素对肠道内甜味传导相关的信号分子表达的影响. 实验结果发现,卵巢切除后的小鼠,血液内雌激素的含量明显降低,体重显著性增加; 并且降低了十二指肠内T1R3的表达,对T1R2、TRPM5也有一定的抑制作用,暗示雌激素在小鼠肠道味觉感知中起着重要作用.Estrogen has been known to be associated with the perception of sweetness,which can modify people’s intake of carbohydrates by affecting the perception of sweetness. However,the mechanism of how is the estrogen involved in the intestinal taste processing has not been fully understood. In this study,we have established the mouse model of estrogen deficiency,by the means of ovariectomy. The consequent modifications of the sweet taste transduction related signal expression have been analyzed on molecular and protein levels in intestine with qRT-PCR and Western blot technique. The results show that,with ovaries removed mice,estrogen levels in blood decreased markedly and the body weight increased significantly. Meanwhile,the expression of the duodenum T1R3 has been reduced,and those of T1R2 and TRPM5 have been inhibited to some extend as well. This study suggests that the estrogen plays an important role in mouse intestinal taste perception
References
[1] bezenconc,furholza,raymondf,etal.murineintestinalcellsexpressingtrpm5aremostlybrushcellsandexpressmarkersofneuronalandinflammatorycells[j].jcompneurol,2008,509(5):514-525. [2] fryeca,crystals,wardkd,etal.menstrualcycleanddietaryrestraintinfluencetastepreferencesinyoungwomen[j].physiolbehav,1994,55(3):561-567. [3] careydg,jenkinsab,campbelllv,etal.abdominalfatandinsulinresistanceinnormalandoverweightwomen:directmeasurementsrevealastrongrelationshipinsubjectsatbothlowandhighriskofniddm[j].diabetes,1996,45(5):633-638. [4] naaza,zakroczymskim,heinep,etal.effectofovariectomyonadiposetissueofmiceintheabsenceofestrogenreceptoralpha(eralpha):apotentialroleforestrogenreceptorbeta(erbeta)[j].hormmetabres,2002,34(11/12):758-763. [5] stubbinsre,holcombvb,hongj,etal.estrogenmodulatesabdominaladiposityandprotectsfemalemicefromobesityandimpairedglucosetolerance[j].eurjnutr,2012,51(7):861-870. [6] nelsong,hoonma,chandrashekarj,etal.mammaliansweettastereceptors[j].cell,2001,106(3):381-390. [7] janghj,kokrashviliz,theodorakismj,etal.gut-expressedgustducinandtastereceptorsregulatesecretionofglucagon-likepeptide-1[j].procnatlacadsciusa,2007,104(38):15069-15074. [8] margolskeerf,dyerj,kokrashviliz,etal.t1r3andgustduciningutsensesugarstoregulateexpressionofna+ -glucosecotransporter1[j].procnatlacadsciusa,2007,104(38):15075-15080. [9] wonggt,gannonks,margolskeerf.transductionofbitterandsweettastebygustducin[j].nature,1996,381(6585):796-800. [10] dotsoncd,ropersd,spectorac.plcbeta2-independentbehavioralavoidanceofprototypicalbitter-tastingligands[j].chemsenses,2005,30(7):593-600. [11] damaks,rongm,yasumatsuk,etal.trpm5nullmicerespondtobitter,sweet,andumamicompounds[j].chemsenses,2006,31(3):253-264. [12] glendinningji,bloomld,onishim,etal.contributionofalpha-gustducintotaste-guidedlickingresponsesofmice[j].chemsenses,2005,30(4):299-316. [13] brixellr,monteilh-zollermk,ingenbrandtcs,etal.trpm5regulatesglucose-stimulatedinsulinsecretion[j].pflugersarch,2010,460(1):69-76. [14] mclaughlinsk,mckinnonpj,margolskeerf.gustducinisataste-cell-specificgproteincloselyrelatedtothetransducins[j].nature,1992,357(6379):563-569. [15] busquesm,kerstetterje,geibeljp,etal.l-typeaminoacidsstimulategastricacidsecretionbyactivationofthecalcium-sensingreceptorinparietalcells[j].amjphysiolgastrointestliverphysiol,2005,289(4):g664-g669. [16] hirat,nakajimas,etoy,etal.calcium-sensingreceptormediatesphenylalanine-inducedcholecystokininsecretioninenteroendocrinestc-1cells[j].febsj,2008,275(18):4620-4626. [17] dyerj,salmonks,zibrikl,etal.expressionofsweettastereceptorsofthet1rfamilyintheintestinaltractandenteroendocrinecells[j].biochemsoctrans,2005,33(pt1):302-305. [18] bezenconc,lecoutrej,damaks.taste-signalingproteinsarecoexpressedinsolitaryintestinalepithelialcells[j].chemsenses,2007,32(1):41-49. [19] hoferd,puschelb,drenckhahnd.tastereceptor-likecellsintheratgutidentifiedbyexpressionofalpha-gustducin[j].procnatlacadsciusa,1996,93(13):6631-6634. [20] hassn,schwarzenbacherk,breerh.aclusterofgustducin-expressingcellsinthemousestomachassociatedwithtwodistinctpopulationsofenteroendocrinecells[j].histochemcellbiol,2007,128(5):457-471. [21] carrdb,utzschneiderkm,hullrl,etal.intra-abdominalfatisamajordeterminantofthenationalcholesteroleducationprogramadulttreatmentpanelⅲcriteriaforthemetabolicsyndrome[j].diabetes,2004,53(8):2087-2094. [22] ferraracm,lynchna,nicklasbj,etal.differencesinadiposetissuemetabolismbetweenpostmenopausalandperimenopausalwomen[j].jclinendocrinolmetab,2002,87(9):4166-4170. [23] tchernofa,despresjp.sexsteroidhormones,sexhormone-bindingglobulin,andobesityinmenandwomen[j].hormmetabres,2000,32(11/12):526-536. [24] dosreiscm,demelonr,meirelleses,etal.bodycomposition,visceralfatdistributionandfatoxidationinpostmenopausalwomenusingoralortransdermaloestrogen[j].maturitas,2003,46(1):59-68. [25] curtisks,stratfordjm,contrerasrj.estrogenincreasesthetastethresholdforsucroseinrats[j].physiolbehav,2005,86(3):281-286. [26] lix,staszewskil,xuh,etal.humanreceptorsforsweetandumamitaste[j].procnatlacadsciusa,2002,99(7):4692-4696. [27] adlere,hoonma,muellerkl,etal.anovelfamilyofmammaliantastereceptors[j].cell,2000,100(6):693-702. [28] maceoj,affleckj,pateln,etal.sweettastereceptorsinratsmallintestinestimulateglucoseabsorptionthroughapicalglut2[j].jphysiol,2007,582(pt1):379-392. [29] maceoj,listern,morgane,etal.anenergysupplynetworkofnutrientabsorptioncoordinatedbycalciumandt1rtastereceptorsinratsmallintestine[j].jphysiol,2009,587(pt1):195-210. [30] legallm,tobinv,stolarczyke,etal.sugarsensingbyenterocytescombinespolarity,membranebounddetectorsandsugarmetabolism[j].jcellphysiol,2007,213(3):834-843.
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