本文旨在探讨长期游泳运动对非酒精性脂肪肝(NAFLD)大鼠血清中氨基酸谱的影响. 30只大鼠随机分为高脂组、高脂运动组和正常组. 高脂组和高脂运动组采用高脂饲料喂养. 高脂运动组喂养4周后,进行20周的游泳运动. 24周后,对3组大鼠取肝脏作病理分析,取血测定血清中ALT、AST、GLU、Chol、TG、HDL、LDL、CP,并采用HPLC测定血清中氨基酸谱,3组进行对照分析. 结果显示,高脂运动组较高脂组肝脏的脂肪变性明显好转,血清中ALT、AST、GLU、Chol、TG、LDL、CP均趋向于正常. 高脂运动组血清中天冬氨酸、丝氨酸、丙氨酸、鸟氨酸、酪氨酸、蛋氨酸、赖氨酸、精氨酸较高脂组显著下降,有统计学意义(P<0.001). 高脂运动组血清中谷氨酸、天冬酰胺、苏氨酸、甘氨酸、缬氨酸、苯丙氨酸较高脂组降低,有显著性差异(P<0.05). 高脂运动组较高脂组支链氨基酸(BCAA)略有上升,芳香族氨基酸(AAA)明显下降,Fischer值(支/芳)明显上升(P<0.05). 研究发现,长期耐力运动通过消耗多余的脂肪使非酒精性脂肪肝肝功能明显好转,血清中氨基酸谱的变化提示长期耐力运动在一定程度上能够纠正蛋白质代谢的紊乱.The purpose of this paper was to study the changes of aminogram in plasma after long-term exercise in non-alcoholic fatty liver disease(NAFLD)rats. Thirty healthy male SD rats were randomly and equally divided into following 3 groups:normal control group(C,fed with standard diet),high fat diet fed control group(HC)and long-term exercise(HE). The rats in group HC were fed with high-fat diet without exercise for 4 weeks,then with 90 min unloaded swimming 5 times per week. The whole duration of the study were 24 weeks,the serum concentration of free amino-acids and ALT,AST,Glu,Chol,TG,HDL,LDL,CP,the morphological changes of liver was detected. Results:ALT,AST,Glu,Chol,TG,HDL,LDL,CP were increased in HC group,while AST,GLU,LDL decreased,and ALT,CHOL,TG decreased significantly(P<0.05 and P<0.01)respectively in HE group. HDL was increased in HE group comparing with HC group. The steatosis was higher in HC group,but was lower in HE group(P<0.05). The Asp,Cir,Ser,Ala,Tyr,Met,Lys,Arg in the serum of HE group were significantly lower than that in HC group(P<0.01),while Glu,Asn,Thr,Gly,Val,Phe in serum lower than that(P<0.05). The levels of most other amino acids tended to decreased in HE group although there was no significant difference compared with HC group(P>0.05). The content of branched-chain amino acid(BCAA)was little lower(P>0.05)but aromatic amino acid(AAA)was lower in HE group(P<0.05)than that in HC group,Fisher ratio was reduced(P<0.05). Our result indicate that increased fat oxidation during long-term exercise could benenif for liver function. The changed of serum aminogram show long-term can correct the disoder of protein metabolism in some degree
