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OALib Journal期刊
ISSN: 2333-9721
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胞外高铁肌红蛋白还原酶电子传递活性位点的研究
, PP. 59-64
Keywords: 高铁肌红蛋白还原酶,电子传递,nadh,nan3,n-ethylmaleimide,二价金属离子
Abstract:
以nadh为反应体系的启动剂,研究猪心肌提取液中高铁肌红蛋白还原酶(memtbase)活性的表达,实验结果证实酶活性的表达依赖nadh启动,且012mmnadh浓度可充分启动memtbase活性的表达.研究还表明,atp可全程抑制memtbase呼吸链电子的传递,抑制酶的活性;nan3通过阻断呼吸链第三位点电子传递来抑制酶活性的表达;而n-ethylmaleimide能与memtbase官能团巯基(-sh)瞬间结合并快速使酶活性丧失;二价金属离子能干扰活性的表达,zn2+可促进memtbase活性的表达;而cu2+和mg2+均抑制酶活性的表达,且mg2+抑制强度大于cu2+.金属螯合剂edta可掩蔽自由金属离子对memtbase酶促反应的干扰,改善酶活性的表达.
| [1] | ariharak,cassensrg,eta.llocalizationofmetmyoglobin-reducingenzyme(nadh-cytochromeb5reductase)systemcomponentsinbovineskeletalmuscle[j].meatscience,1995,39:205-213.
|
| [2] | 汤祥明,金邦荃,曹鹏,等.猪心肌中高铁肌红蛋白的提取和纯化[j].畜牧与兽医,2006,38(7):34-36.
|
| [3] | bekhitad,geesinkgh,ilianma,eta.lparticulatemetmyoglobinreducingactivityanditsrelationshipwithmeatcolor[j].jagriculturalandfoodchemistry,2003,51(20):6026-6035.
|
| [4] | warrisspd.meatscience:anintroductorytext[m].newyork:cabipublishing,2000.
|
| [5] | 王镜岩,朱圣庚,徐长法.生物化学[m].北京:高等教育出版社,2002:128-129,140-141.
|
| [6] | deanrw,ballco.analysisofthemyoglobinfractionsonthesurfaceofbeefcut[j].foodtechnology,1960,14:271-285.
|
| [7] | mikkelsena,juncherd,skibstedlh.metmyoglobinreductaseactivityinovinem.longissimusdorsimuscle[j].meatscience,1999,51:155-161.
|
| [8] | ledwardda.colourofrawandcookedmeat.thechemistryofmuscle-basedfoods[m].cambridge:theroyalsocietyofchemistry,1992:33-68.
|
| [9] | tangjial,icameronfaustma,lricharda,eta.lmitochondrialreductionofmetmyoglobin:dependenceontheelectrontransportchain[j].journalofagriculturalandfoodchemistry,2005,53(13):5449-5455.
|
| [10] | 王玮,汤祥明,金邦荃.高铁肌红蛋白、高铁肌红蛋白还原酶与冷鲜肉肉色稳定性的研究[j].食品科学,2008,29(7):94-97.
|
| [11] | echevarnec,renerrem,labasr.metmyoglobinreductaseactivityinbovinemuscles[j].meatscience,1990,27:161-172.
|
| [12] | bekhitaed,geesinkgh,mortonjd,eta.lmetmyoglobinreducingactivityandcolourstabilityofovinelongissimusmuscle[j].meatscience,2001,57(4):427-435.
|
| [13] | 俞秀玲,朱忠勇.猪肝微粒体nadh-细胞色素b5还原酶的纯化及特性分析[j].生物化学与生物物理学报,1994,26(3):297-302.
|
| [14] | wattsbm,kendrickj,zipsermw,eta.lenzymaticreducingpathwaysinmeat[j].journaloffoodscience,1966,32:855-862.
|
| [15] | pongcy,chioutk,niehfp,eta.lpurificationandcharacterizationofmetmyoglobinreductasefromordinarymuscleofblue-fintuna[j].fisheriesscience,2000,66(3):599-604.
|
| [16] | haglerl,coppesj.metmyoglobinreductaseidentificationandpurificationofareducednicotinamideadeninedinucleotidedependent
|
| [17] | enzymefrombovineheartwhichreducesmetmyoglobin[j].journalofbiologicalchemistry,1979,254(14):6505-6514.
|
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