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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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溴氰菊酯胁迫下小菜蛾差异表达基因的筛选与分析
, PP. 91-95
Keywords: 小菜蛾,抗性遗传,菌落pcr,cdnarda
Abstract:
通过双向cdnarda,即分别以敏感品系为检测子(tester)、抗溴氰菊酯品系为驱赶子(driver)和以抗溴氰菊酯品系为检测子(tester)、敏感品系为驱赶子(driver)筛选2种品系中的差异表达基因,初步分析小菜蛾敏感品系和抗性品系之间的遗传差异.经过3轮消减杂交后,将所得的差异片段克隆于pmd18-t载体,氨苄筛选阳性克隆,经菌落pcr鉴定后送样测序.得到2条序列与genbank数据库中的部分已知序列有一定同源性,其中1条序列与编码s3a蛋白基因有较高的同源性.
| [1] | hubankm,schatzdg.identifyingdifferencesinmrnaexpressionbyrepresentationaldifferenceanalysisofcdna[j].nucleicacidsresearch,1994,22(25):5640-5648.
|
| [2] | elkelammens,pieter-janceyssens,marleenvoet,kirstenhertveldt,roblavigne,guidovolckaert.representationaldifferenceanalysis(rda)ofbacteriophagegenomes[j].journalofmicrobiologicalmethods,2009,77(2):207-213.
|
| [3] | 王淑珍,程罗根,陈之浩,等.小菜蛾澳氰菊醋抗性和敏感种群遗传差异的cdnarda分析[j].植物保护学报,2005,32(1):63-66.
|
| [4] | 张晓飞,程罗根,陈之浩,等.小菜蛾对杀虫双抗性遗传的rda分析[j].南京师大学报:自然科学版,2003,26(1):78-82.
|
| [5] | 张海,程罗根,陈之浩,等.抗杀虫双品系小菜蛾抗药性的rda分析[j].江苏农业科学,2004,5:46-50.
|
| [6] | westermannp,heumannw,bommerua.cross-linkingofinitiationfactorelf-2toproteinsofthesmallsubunitofratliverribosomes[j].febslett,1979,97:101-104.
|
| [7] | tushinskirj,warnerjr.ribosomalproteinsaresynthesizedpreferentiallyincellscommencinggrowth[j].jcellphys-io,l1982,112:128-135.
|
| [8] | tolandr,hersheyjwb,trautrr.cross-linkingofeukaryoticinitiationfactorelf-3tothe40sribosomalsubunitfromrabbitreticulocytes[j].biochimie,1983,65(7):427-436.
|
| [9] | 孙阿萍,吕晓平,张秋迟,等.细胞凋亡过程中核糖体蛋白s3a水平变化的研究(一)[j].黑龙江医药科学,2001,24(3):1-4
|
| [10] | lixian,zhangshicu,iliuzhenhu,ieta.lribosomalproteingeness23andl35fromamphioxusbranchiostomabelcheritsingtauense:identificationandcopynumber[j].actabiochimicaetbiophysicasinica,2005,37(8):573-579.
|
| [11] | mohanm,gujargt.localvariationinsusceptibilityofthediamondbackmoth,plutellaxylostella(linnaeus)toinsect-icidesandroleofdetoxificationenzymes[j].cropprotection,2003,22(3):495-504.
|
| [12] | lisitsynna,lisitsynnm,wiglermh.cloningthedifferencebetweentwocomplexgenomes[j].science,1993,259:946-951.
|
| [13] | cluogen,wshuzhen,czhihao,eta.lcdnarepresentationaldifferenceanalysisofthedeltamelthrin-resistantand-susceptiblepopulationsindiamondbackmoth(plutellaxylostellal.)[j].japplentomo,l2005,129(9/10):515-520.
|
| [14] | songd,sakamotos,taniguchit.inhibitionofpoly(adp-ribose)polymeraseactivitybybc-l2inassociationwithther-ibosomalproteins3a[j].biochemistry,2002,41(3):929-934.
|
| [15] | 徐家萍,陈克平,姚勤,等.利用荧光差异显示技术分离的家蚕抗npv相关基因s3a[j].昆虫学报,2005,48(3):347-352.
|
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