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OALib Journal期刊
ISSN: 2333-9721
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利用rna干扰技术创制高直链淀粉马铃薯新材料
Keywords: 转基因马铃薯,sbe基因,rna干扰,直链淀粉
Abstract:
直链淀粉由于其独特的理化性质,在工业上有着广泛的应用.郭志鸿[1]等利用rna干扰技术抑制马铃薯中两类淀粉分支酶亚基基因sbe1和sbe2的表达,有效提高了马铃薯的直链淀粉含量.本研究利用rna干扰技术,构建马铃薯sbeⅰ(thendogenesisstarchbranchingenzymeⅰ)和sbeⅱ(theendogenesisstarchbranchingenzymeⅱ)单基因以及sbe玉和sbe域双基因的rna干扰双元表达质粒载体.采用农杆菌介导法将重组基因
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