聚(3,4-乙烯基二氧噻吩)/碳纳米管复合膜修饰电极的电化学制备及其用于8-羟基-2\’-脱氧鸟嘌呤核苷的检测
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Keywords: 8-羟基-2’-脱氧鸟嘌呤核苷,聚(3,4-乙烯基二氧噻吩),碳纳米管,导电聚合物,尿酸
Abstract:
通过恒电流电解一步法在玻碳电极表面制备了聚(3,4-乙烯基二氧噻吩)/碳纳米管复合膜,实验结果表明,该复合膜修饰电极综合了聚(3,4-乙烯基二氧噻吩)(pedot)和碳纳米管(cnts)两者的优点,对8-羟基-2’-脱氧鸟嘌呤核苷(8-oh-dg)的氧化具有明显的增强作用,较好地抑制尿酸的干扰,而且具有很好的重现性和稳定性.在0.1mol/lph9.0的磷酸盐缓冲溶液(pbs)中,8-oh-dg的氧化峰电流与其浓度在0.014～14.0μmol/l和14.0～56.0μmol/l两个范围内成良好的线性关系,检出限可达35nmol/l(s/n=3).
| [1] | kasaih．analysisofaformofoxidativednadamage，8-hydroxy-2’-deoxyguanosine，asamarkerofcellularoxidativestressduringcarcinogenesis[j]．mutatres，1997，387(3):147-163．
|
| [2] | toraasonm．8-hydroxydeoxyguanosineasabiomarkerofworkplaceexposures[j]．biomarkers，1999，4(1):3-26．
|
| [3] | lics，wuky，chang-chiengp，etal．analysisofoxidativednadamage8-hydroxy-2’-deoxyguanosineasabiomarkerofexposurestopersistentpollutantsformarinemammals[j]．environscitechnol，2005，39(8):2455-2460．
|
| [4] | shigenagama，amesbn．assayfor8-hydroxy-2-deoxyguanosine:abiomarkerofinvivooxidatvednadamage[j]．freeradbiolmed，1991，10(3/4):211-216．
|
| [5] | floydra，watsonjj，wongpk，etal．hydroxylfreeradicaladductofdeoxyguanosine:sensitivedetectionandmechanismsofformation[j]．freeradrescommun，1986，1(3):163-172．
|
| [6] | lofts，vistisenk，ewertzm，etal．oxidativednadamageestimatedby8-hydroxy-deoxy-8-hydroxy-deoxyguanosineexcretioninhumans:influenceofsmoking，genderandbodymassindex[j]．carcinogenesis，1992，13(12):2241-2247．
|
| [7] | lith，jiawl，wanghs，etal．electrochemicalperformanceof8-hydroxy-2’-deoxyguanosineanditsdetectionatpoly(3-methylthiophene)modifiedglassycarbonelectrode[j]．bisens，bioelectron，2007，22:1245-1250．
|
| [8] | 高盐生，董江庆，徐晓燕．纳米技术在生物传感器中的研究应用[j]．江苏化工，2008，36(3):4-6．
|
| [9] | merza．bardaj．astablesurfacemodifiedplatinumelectrodepreparedbycoatingwithelectroactivepolymer[j]．jamchemsoc，1978，100(10):3222-3223．
|
| [10] | 徐幸梓，王必本，刘天模．碳纳米管性能的研究进展[j]．材料导报，2001，15(11):44-45．
|
| [11] | zekram，johanb，andrzejl，etal．poly(3，4-ethylenedioxythiophene)(pedot)dopedwithcarbonnanotubesasion-toelectrontransducerinpolymermembrane-basedpotassiumionselectiveelectrodes[j]．jelectroanalchem，2009，633(1):246-252．
|
| [12] | 鲁文红，刘爱玲，王先良，等．利用高效液相色谱-电化学法检测尿液8-羟基脱氧鸟苷[j]．广东医学，2006，27(12):1802-1803．
|
| [13] | guytonkz，kenslertw．oxidativemechanismsincarcinogenesis[j]．mutatres，1993，49(3):523-526．
|
| [14] | ladk，swenbergja．dnaadducts:biologicalmarkersofexposureandpotentialapplicationstoriskassessment[j]．mutatres，1996，365(1/3):129-146．[8]fragacg，shigenagamk，parkjw，etal．oxidativedamagetodnaduringaging:8-hydroxy-2’-deoxyguanosineinratorgandnaandurine[j]．procnatlacadsciusa，1990，87(12):4533-4537．
|
| [15] | suzukih，inouey，suzukis．changesintheurinaryexcretionlevelof8-hydroxyguaninebyexposuretoreactiveoxygengeneratingsubstances[j]．freeradbiolmed，1995，18(3):431-436．
|
| [16] | wangyh，lij，liuy，etal．fabricationofthedna/poly(3-methylthiophene)compositefilmmodifiedelectrodeanditsapplicationforthestudyonthevoltammetricbehavioranddeterminationof8-hydroxy-2’-deoxyguanosine[j]．scichinaserb-chem，2009，52(11):2006-2012．
|
| [17] | iijimas．helicalmicrotubulesofgraphiticcarbon[j]．nature，1991，354:56-58．
|
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