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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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ggpps多克隆抗体的制备与鉴定
, PP. 94-98
Keywords: ggpps,原核表达,融合蛋白,多克隆抗体
Abstract:
构建人香叶基香叶基二磷酸合成酶基因(ggpps)原核表达载体,制备兔抗人ggpps多克隆抗体.利用dna重组技术构建原核表达载体pgex-4t-ggpps,诱导表达ggpps融合蛋白,分离纯化该融合蛋白后,作为免疫原免疫家兔制备多克隆抗体.成功构建了pgex-4t-1-ggpps原核表达载体,转化rosetta菌株后可高效表达ggpps融合蛋白,成功制备ggpps多克隆抗体,间接elisa法测定抗体效价为1:32000,并且抗体具有良好特异性.成功克隆ggpps基因并制备了特异性的兔抗人ggpps多克隆抗体,为进一步研究ggpps的功能奠定了基础.
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