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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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2型猪链球菌中国强毒株05zyh33ofsn-片段基因敲除株的构建
, PP. 87-92
Keywords: 2型猪链球菌,ofs,基因敲除株
Abstract:
通过生物信息学分析2型猪链球菌(streptococcussuisserotype2,s.suis2)中国强毒株05zyh33的基因组,预测并发现猪链球菌血清浑浊因子(opacityfactorofs.suis,ofs)的编码基因ofs.为了构建ofsn-末端片段ofs37-683的基因敲除株,首先构建中间为壮观霉素抗性基因,两侧为ofs37-683上、下游同源序列的基因敲除质粒,并对该质粒进行pcr和酶切鉴定.根据同源重组的原理,通过电转化方法筛选得到ofs37-683基因敲除株.pcr、测序和rt-pcr分析结果均显示ofs37-683已完全被壮观霉素抗性基因替代,证实ofs37-683基因敲除株构建成功.该敲除株的获得为进一步研究ofs在猪链球菌2型致病机制中的作用奠定基础.
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