|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
嗜麦芽寡养单胞菌(stenotrophomonasmaltophilia)r551-3四环素抗性消除的研究
, PP. 84-89
Keywords: 嗜麦芽寡养单胞菌,吡虫啉,电穿孔,抗性消除,四环素
Abstract:
嗜麦芽寡养单胞菌可共代谢降解烟碱类农药吡虫啉,并且对多种抗生素具有抗性.本文通过电穿孔法消除了该菌的四环素抗性,获得了四环素敏感型菌株r551-3tcr-,可以用作遗传转化系统的受体菌,能被转化吸收遗传载体质粒pjb866h::ndhsl,构成一对很好的嗜麦芽寡养单胞菌遗传转化系统.同时hplc结果表明所获得的四环素敏感型菌株羟基化吡虫啉的活性并未发生改变,为进一步开展敲除和回补共代谢途径关键酶的研究工作奠定了基础.
| [1] | zhangl,lixz,poolek.smedefmultidrugeffluxpumpcontributestointrinsicmultidrugresistanceinstenotrophomonasmaltophilia[j].antimicrobagentschemother,2001,45(12):3497-3503.
|
| [2] | blancom,kadleck,gutierrezmartincb,eta.lnucleotidesequenceandtransferpropertiesoftwonoveltypesofact-inobacilluspleuropneumoniaeplasmidscarryingthetetracyclineresistancegenetet(h)[j].jantimicrobchemother,2007,60(4):864-867.
|
| [3] | heerydm,powellr,gannonf,eta.lcuringofaplasmidfrome.coliusinghigh-voltageelectroporation[j].nucleicacidsres,1989,17(23):10131.
|
| [4] | 黄必旺,黄志鹏,关雄.电穿孔法用于大肠杆菌和苏云金杆菌的转化及质粒消除[j].福建农业大学学报,1999,28(1):43-46.
|
| [5] | 陈乃用.电穿孔法在细菌质粒转化中的应用[j].微生物学通报,1991,18(2):97-103.
|
| [6] | yangy,yuans,chent,eta.lcloning,heterologousexpression,andfunctionalcharacterizationofthenicotinatedehydrogenasegenefrompseudomonasputidakt2440[j].biodegradation,2009,20:541-549.
|
| [7] | mckayga,woodsde,macdonaldkl,eta.lroleofphosphoglucomutaseofstenotrophomonasmaltophiliainlipopolysaccharidebiosynthesis,virulence,andantibioticresistance[j].infectimmun,2003,71(6):3068-3075.
|
| [8] | furushitam,okamotoa,maedat,eta.lisolationofmultidrug-resistantstenotrophomonasmaltophiliafromculturedye-llowtail(seriolaquinqueradiata)fromamarinefishfarm[j].applenvironmicrobio,l2005,71(9):5598-5600.
|
| [9] | gouldvc,avisonmb.smedef-mediatedantimicrobialdrugresistanceinstenotrophomonasmaltophiliaclinicalisolateshavingdefinedphylogeneticrelationships[j].jantimicrobchemother,2006,57(6):1070-1076.
|
| [10] | changll,chenhf,changcy,eta.lcontributionofintegrons,andsmeabcandsmedefeffluxpumpstomultidrugresistanceinclinicalisolatesofstenotrophomonasmaltophilia[j].jantimicrobchemother,2004,53(3):518-521.
|
| [11] | hansenlh,johannesene,burmollem,eta.lplasmid-encodedmultidrugeffluxpumpconferringresistancetoolaquindoxinescherichiacoli[j].antimicrobagentschemother,2004,48(9):3332-3337.
|
| [12] | 孙二琳.嗜麦芽寡养单胞菌耐药机制的研究[j].中国抗生素杂志,2003,28(7):445-448.
|
| [13] | vatteronil,pirasa,simis,eta.lanalysisofelectroporation-inducedgeneticdamagesinv79/ap4chinesehamstercells[j].mutatres,1993,291(3):163-169.
|
| [14] | higginsdr,strathernjn.electroporation-stimulatedrecombinationinyeast[j].yeast,1991,7(8):823-831.
|
| [15] | daiyj,chent,gef,eta.lenhancedhydroxylationofimidaclopridbystenotrophomonasmaltophiliauponadditionofsucrose[j].applmicrobiolbiotechno,l2007,74(5):995-1000.
|
| [16] | daiyj,yuans,gef,eta.lmicrobialhydroxylationofimidaclopridforthesynthesisofhighlyinsecticidalolefinimidacloprid[j].applmicrobiolbiotechno,l2006,71(6):927-934.
|
| [17] | 杨顺瑛,姬微微,戴亦军,等.嗜麦芽寡养单胞菌菌株间羟基化烟碱类杀虫剂吡虫啉的机制差异[j].自然科学进展,2008,18(10):1163-1168.
|
| [18] | chent,daiyj,dingjf,eta.ln-demethylationofneonicotinoidinsecticideacetamipridbybacteriumstenotrophomonasmaltophiliacgmcc1??1788[j].biodegradation,2008,19(5):651-658.
|
| [19] | zhaoyj,daiyj,yucg,eta.lhydroxylationofthiaclopridbybacteriumstenotrophomonasmaltophiliacgmcc1??1788[j].biodegradation,2009,20(6):761-768.
|
| [20] | 耿毅,汪开毓,陈德芳,等.嗜麦芽寡养单胞菌研究进展[j].动物医学进展,2006,27(5):28-31.
|
| [21] | lescatm,calteaua,hoedec,eta.lamodulelocatedatachromosomalintegrationhotspotisresponsibleforthemult-idrugresistanceofareferencestrainfromescherichiacoliclonalgroupa[j].antimicrobagentschemother,2009,53(6):2283-2288.
|
| [22] | sanmillana,escuderoja,gutierrezb,eta.lmultiresistanceinpasteurellamultocidaismediatedbycoexistenceofsmallplasmids[j].antimicrobagentschemother,2009,53(8):3399-3404.
|
| [23] | jahagirdars,patwardhanr,dhakephalkarpk.curingplasmid-mediatedvancomycinresistanceinstaphylococcusaureususingherbalnaphthoquinones[j].jhospinfect,2008,70(3):289-291.
|
| [24] | nib,duz,guoz,eta.lcuringoffourdifferentplasmidsinyersiniapestisusingplasmidincompatibility[j].lettapplmicrobio,l2008,70(3):289-291.
|
| [25] | 杨瑶,袁生,戴亦军,等.烟酸羟基化菌株睾酮丛毛单胞菌ja1抗性质粒的消除[j].南京师大学报:自然科学版,2007,30(2):77-80.
|
| [26] | 李苌清,周岐新,凌保东.耐药性质粒消除的研究概况与展望[j].川北医学院学报,2003,18(2):168-171.
|
| [27] | 项裕财.r质粒的紫外消除试验改进和体会[j].皖南医学院学报,2001,20(2):154.
|
| [28] | 娄恺,班睿,赵学明.细菌质粒的消除[j].微生物学通报,2002,29(5):99-103.
|
| [29] | 刘渠,白松涛,叶梅君,等.g+球菌及g-杆菌质粒消除方法的研究[j].中国卫生检验杂志,1998,8(5):275-278.
|
| [30] | 龙海英,刘横川,方梅.质粒消除方法及质粒消除效果的评价研究[j].中国卫生检验杂志,2007,17(3):413-415.
|
| [31] | 萨姆布鲁克j,拉w.分子克隆实验指南[m].2版.黄培堂,译.北京:科学出版社,1999.
|
| [32] | avisonmb,higginscs,vonheldreichcj,eta.lplasmidlocationandmolecularheterogeneityofthel1andl2betalactamasegenesofstenotrophomonasmaltophilia[j].antimicrobagentschemother,2001,45(2):413-419.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
