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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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长型肌球蛋白轻链激酶4ig结构域的表达及单克隆抗体的制备
, PP. 124-128
Keywords: mlck4ig,蛋白表达,单克隆抗体
Abstract:
长型肌球蛋白轻链激酶(longmyosinlightchainkinase,l-mlck)及其分子内结构域的功能还不清楚.在研究其功能时,特异性抗体是一基本工具,但目前国际上还没有制备成功特异性较好的抗体.为此,我们先在大肠杆菌e.colibl21中重组表达了鸡l-mlck的4ig蛋白片段(mlck4ig),蛋白经纯化后作为抗原免疫小鼠.在小鼠血清中检测到特异性抗体的基础上,建立了2株杂交瘤细胞株,获得抗鸡mlck4ig的单克隆抗体.该抗体可以特异与鸡l-mlck和鸡mlck4ig反应.本文并对该抗体的特性及应用进行了讨论.结果表明,我们部分解决了l-mlck的抗体缺乏问题,为研究l-mlck及mlck4ig片段的功能提供了重要工具.
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