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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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重组猪α-干扰素的纯化及生化性质鉴定
, PP. 97-100
Keywords: 重组猪α-干扰素(poifn-α),亲和层析,离子交换层析,糖基化,生化性质
Abstract:
对巴斯德毕赤酵母高效分泌表达的猪α-干扰素(poifn-α)纯化工艺和纯化后重组蛋白的部分生化特性进行了研究,结果表明:猪α-干扰素(poifn-α)发酵液经离心、透析、过滤处理之后,依次利用亲和层析和离子交换层析使目的蛋白得到了纯化.经n端氨基酸测序,western-blot,对酸、热、巯基乙醇的稳定性和糖基化程度等检测后发现,所表达的猪α-干扰素n端氨基酸序列(前5个)正确,对酸和热基本稳定,二硫键对目的蛋白的活性至关重要,没有发现目的蛋白的糖基化.
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