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OALib Journal期刊
ISSN: 2333-9721
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肿瘤特异性溶瘤腺病毒载体的构建及其在肿瘤细胞中特异性的表达
, PP. 92-96
Keywords: 基因治疗,溶瘤腺病毒,肿瘤,端粒酶启动子
Abstract:
为构建一种由人端粒酶逆转录酶启动子(htertp)调控的能够在肿瘤细胞中特异性增殖的新型肿瘤基因治疗溶瘤腺病毒载体系统,利用基因重组技术将htertp插入5型腺病毒e1a基因上游,构建肿瘤特异性增殖腺病毒adsu,使其携带绿色荧光蛋白报告基因.同时构建增殖缺陷型腺病毒ad-egfp作为对照.通过westernblot分析表明该特异性增殖腺病毒adsu-egfp在肿瘤细胞中特异性表达eia.而通过荧光显微镜观察两者增殖实验发现,adsu-egfp在正常成纤维细胞株bj及原代子宫成纤维细胞中无增殖,在肝癌细胞株mhcc和肺癌细胞株a549中,则可见病毒增殖并裂解细胞的现象.由此可认为成功构建了肿瘤基因治疗的特异性溶瘤腺病毒载体系统adsu,该系统能够在肿瘤细胞中特异性增殖并表达所携带的外源基因,这对肿瘤的定向基因治疗有着重要意义.
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