|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
fitc标记的gstp1蛋白在鼠巨噬细胞中的定位
, PP. 105-110
Keywords: gstp1,fitc,薄层色谱扫描,激光共聚焦,流式分析
Abstract:
gstp1(gstp)是人体内谷胱甘肽-s-转移酶(gst)的一种重要的活性亚型,属于机体内源性蛋白,不会引起免疫原反应,在维持细胞的正常机能中发挥着重要作用,也是一种具有临床应用价值的抗氧化剂.本实验室在研究中首先发现gstp1具有明显的抗炎作用,由此我们设想可以通过增加外源性的gstp1蛋白来提高其在机体内的水平,以此增强机体的抗炎能力.本实验将薄层色谱扫描和sds-page相结合用于fitc标记蛋白质的检测,使用激光共聚焦显微镜和流式分析相结合的方法研究了外源gstp1蛋白在raw26417巨噬样细胞和鼠腹腔巨噬细胞中的跨膜转位,结果显示外源gstp1蛋白能够进入上述细胞.该研究为gstp1蛋白作为直接使用的药物在抗肿瘤、抗炎、抗氧化等领域的应用提供了实验基础.
| [1] | hawigerj.innateimmunityandinflammation:atranscriptionalparadigm[j].immunolres,2001,23(2):99-109.
|
| [2] | 陈敏英.全身炎症反应综合征及治疗进展[j].中国实用外科杂志,2002,22(12):766-768.
|
| [3] | cunneen,jcartwrightm.thepuzzleofsepsis:fittingthepiecesoftheinflammatoryresponsewithtreatment[j].aacnclinissues,2004,15(1):18-44.
|
| [4] | 张顺财.内毒素基础与临床[m].北京:科学出版社,2002.
|
| [5] | 姜勇等.lps介导细胞激活的信号转导:从cd14到p38mapk通路的研究[j].生理科学进展,1999,30(1):29-34.
|
| [6] | danyellemtownsend1,kennethdtew.theroleofglutathione-s-transferaseinant-icancerdrugresistance[j].oncogene,2003,22(20):7369-7375.
|
| [7] | pickettcb,luay.glutathiones-transferase:genestructure,regulation,andbiologicalfunction[j].annurevbiochem,1989,58:743-764.
|
| [8] | adlerv,yinz,fuchss,eta.lregulationofjnksignalingbygstp[j].emboj.1999,18(5):1321-1334.
|
| [9] | davidleaton,theokbammler.concisereviewoftheglutathiones-transferasesandtheirsignificancetotoxicology[j].
|
| [10] | toxicologysciences,1999,49(2):156-164.
|
| [11] | richardc,strange,monicaaspiter,ieta.lglutathione-s-transferasefamilyofenzymes[j].mutationresearch,2001,482(1):21-26.
|
| [12] | wuy,fany,xueb,eta.lhumanglutathiones-transferasep1-1interactswithtraf2andregulatestraf2)ask1signals[j].oncogene,2006,25(42):1-14.
|
| [13] | xueb,wuy,yinz,eta.lregulationoflipopolysaccharide-inducedinflammatoryresponsebyglutathiones-transferasep1inraw26417cells[j].febslett,2005,579(19):4081-4087.
|
| [14] | ruthannveach,danyaliu,shanyao,eta.lreceptor/transporter-independenttargetingoffunctionalpeptidesacrosstheplasmamembrane[j].jbiolchem,2004,279(12):11425-11431.
|
| [15] | hastingsrh,folkessonhg,petersenv,eta.lcellularuptakeofalbuminfromlungsofanethetizedrabbits[j].amjphysio,l1995,269(11):453-462.
|
| [16] | oakley1aj,lobellom,nuccetellim,eta.lthelig2andin(non2substrate)bindingsiteofhumanpiclassglutathione
|
| [17] | transferaseislocatedintheelectrophilebindingsite(h2site)[j].jmolbio,l1999,291(4):913-926.
|
| [18] | reinemerp,dirrhw,ladensteinr,eta.lthree2di2mensionalstructureofclasspiglutathione2s2trans2ferasefromhumanplacentaincomplexwiths2hexyl2glutathioneat2.8aresolution[j].jmolbio,l1992,227(4):214-226.
|
| [19] | wuy,shenj,yinz.expression,purificationandfunctionalanalysisofhexahistidine-taggedhumanglutathiones-transferasep1-1anditscysteinylmutants[j].theproteinjourna,l2007,26(6):359-370.
|
| [20] | daewoongjo1,danyaliu1.intracellularproteintherapywithsocs3inhibitsinflammationandapoptosis[j].naturemedicine,2005,11(8):892-898.
|
| [21] | paddocksw.principleandpracticesoflaserscanningconfocalmicroscopy[j].molbiotechno,l2000,16(2):127-149.
|
| [22] | 金伯泉.细胞和分子免疫学[m].北京:科学出版社,2001.
|
| [23] | hitoshisate,yuichisugiyama.importanceofreceptor-mediatedendocytosisinpeptidedeliveryandtargeting:kineticaspects[j].advanceddrugdeliveryreviews,1996,19(3):445-467.
|
| [24] | ehrenreichba,cohnza.theuptakeanddigestionofiodinatedhumanserumalbuminbymacrophagesinvitro[j].jexpmed,1967,126(23):941-958.
|
| [25] | catherinelombry,cynthiabosquillon.confocalimagingofratlungsfollowingintratrachealdeliveryofdrypowdersorsolutionsoffluorescentprobes[j].journalofcontrolledrelease,2002,83(3):331-341.
|
| [26] | raijmakersmt,roelofshm,steegersea,eta.lglutathioneandglutathiones-transferasesa1-1andp1-1inseminalplasmamayplayaroleinprotectingagainstoxidativedamagetospermatozoa[j].fertilsteri,l2003,79(1):169-172.
|
| [27] | nijhof,fwa,muldertp,eta.leffectsofconsumptionofbrusselssproutsonplasmaandurinaryglutathiones-transferaseclass-alphaand-piinhumans[j].carcinogenesis,1995,16(4):955-957.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
