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OALib Journal期刊
ISSN: 2333-9721
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中国梨s？rnase基因启动子的tail？pcr克隆及功能预测
DOI: 10.3969/j.jssn.1000-2006.2010.04.005, PP. 21-25
Keywords: tail？pcr,梨,s？rnase,启动子
Abstract:
利用tail？pcr技术从中国砂梨品种‘金花’、‘懋功’及白梨品种‘鸭梨’基因组dna中分别扩增出长度为854、1448和1137bp的s13-、s12-、s21-rnase基因5′端上游序列，并提交genbank，序列号为hm047239、hm047240、hm047241。经place和plantcare软件顺式调控元件预测发现，这3个启动子均具有典型核心启动子tata盒和caat盒，且在上游均存在光应答调控元件(box4，g？box)、脱落酸应答元件abre及水杨酸应答元件tca？element等，由此可知其可能受光照、脱落酸、水杨酸等多种条件的共同调节。此外，与已知日本梨s2-、s3-、s4-、s5-rnase基因启动子序列比对发现，s？rnase启动子tata盒上游存在一约200bp的同源区域。以meg4.0构建系统发育树表明，梨属和苹果属s？rnase等位基因多态性可能在亚科的分化之前就已形成。
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