|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
橡胶树胶乳c-乳清死皮相关蛋白的鉴定及分析
DOI: 10.3969/j.jssn.1000-2006.2012.05.006, PP. 37-41
Keywords: 橡胶树死皮,胶乳c-乳清,蛋白鉴定,双向凝胶电泳,maldi-tofms
Abstract:
橡胶树死皮是影响天然橡胶产量的重要限制因子。通过双向凝胶电泳技术(2-de)研究了橡胶死皮树与健康树胶乳c-乳清蛋白表达谱的差异。以软件分析后共获得31个差异表达的蛋白点，经基质辅助激光解析电离飞行时间质谱(maldi-tofms)分析后，搜索ncbinr数据库，结果有10个蛋白点被成功鉴定，其中6个为未知蛋白，其余4个蛋白分别为chaina,structureofubiquitin？likeprotein,rub1；β？1,3？glucanase；air9protein和flagellarinnerdyneinarmheavychain11。这些蛋白可能在橡胶树死皮发生过程中扮演重要角色。
| [1] | 刘志昕，郑学勤.橡胶树死皮病的发生机理和假说[j].生命科学研究,2002,6(1):83-85.？
|
| [2] | 覃宝祥,胡新文,邓晓东,等.橡胶树死皮病的形成及其机制[j].植物生理学通讯,2005,41(6):827-830.？
|
| [3] | suvachittanontw,wititsuwannakulr.3？hydroxy？3？methylglutaryl？coenzymeasynthasein？heveabrasiliensis？[j].phytochemistry,1995,40（3）:757-761.？
|
| [4] | poscha,chenz,wheelec,etal.characterizationandidentificationoflatexallergensbytwo？dimensionalelectrophoresisandproteinmicrosequencing[j].jallergyclinimmunol,1997,99（3）:385-395.？
|
| [5] | lacrotter,vichitcholchain,chrestinh,etal.proteinmarkerslinkedtothetappingpaneldryness(tpd)of？heveabrasiliensis？[c]//.irrdbworkshopontappingpaneldrynessin？heveabrasiliensis？.haikou:catas,1997.？
|
| [6] | 袁坤，王真辉，丁璇，等.巴西橡胶树树皮蛋白质组学分析体系的构建[j].南京林业大学学报：自然科学版，2009,33(6):146-149.？
|
| [7] | vierstrard，callisj.polypeptidetags,ubiquitousmodifiersforplantproteinregulation[j].plantmolbiol,1999,41:435-442.？
|
| [8] | melchiorf.sumo？nonclassicalubiquitin[j].annurevcelldevbiol,2000,16:591-626.？
|
| [9] | kurepaj,walkerjm,smallej，etal.thesmallubiquitin？likemodifier(sumo)proteinmodificationsystemin？arabidopsis？.accumulationofsumo1and？2conjugatesisincreasedbystress[j].biolchem,2003,278:6862-6872.？
|
| [10] | lid,dengz,chenc,etal.identificationandcharacterizationofgenesassociatedwithtappingpaneldrynessfrom？heveabrasiliensis？latexusingsuppressionsubtractivehybridization[j].bmcplantbiol,2010,10（1）:140-152.？
|
| [11] | venkatachalamp,thulaseedharana,raghothamak.identificationofexpressionprofilesoftappingpaneldryness(tpd)associatedgenesfromthelatexofrubbertree(？heveabrasiliensis？muell.arg.)[j].planta,2007,226:499-515.？
|
| [12] | anurathacs,zenkc,colekc,etal.inductionofchitinasesandβ？1,3？glucanasesin？rhizoctoniasolani？infectedriceplants:isolationofaninfection？relatedchitinasecdnaclone[j].physiolplant,1996,97:39-46.？
|
| [13] | sookmarku,pujode？renaudv,chrestinh,eta1.characterizationofpolypeptidesaccumulatedinthelatexcytosolofrubbertreeaffectedbythetappingpaneldrynesssyndrome[j]．plantcellphysiol，2002，43：1323-1333．？
|
| [14] | blumh,beierh,grossh.improvedsilverstainingofplantproteins,rnaanddnainpolyacrylamidegels[j].electrophoresis,1987,17(8):93-99.？
|
| [15] | fernandezj,gharahdaqhif,mischesm.routineidentificationofproteinsfromsodiumdodecylsulfate？polyacrylamidegelelectrophoresis（sds？page）gelorpolyvinyldifluoridemembranesusingmatrixassistedlaserdesorption/ionization？timeofflight？massspectrometry（maldi？tof？ms）[j].electrophoresis,1998,19（6）:1036-1045.？
|
| [16] | gharahdaghif,weinbergcr,meagherda,etal.massspectrometricidentificationofproteinsfromsilver？stainedpolyacrylamidegel：amethodfortheremovalofsilverionstoenhancesensitivity[j].electrophoresis,1999,20(3)：601-605.？
|
| [17] | mauchf,hadwigerla,bollert.antifungalhydrolasesinpeatissue:i.purificationandcharacterizationoftwochitinasesandtwoβ？1,3？glucanasesdifferentiallyregulatedduringdevelopmentandinresponsetofungalinfection[j].plantphysiol,1988,87:325-333.？
|
| [18] | mauchf,mauch？manib,bollert.antifungalhydrolasesinpeatissue:ii.inhibitionoffungalgrowthbycombinationsofchitinaseandβ？1,3？glucanase[j].plantphysiol,1988,88:936-942.？
|
| [19] | sanchez？ballestamt,zacariasl，granella，etal.β？1,3？glucanasegeneexpressionasamolecularmarkerforpostharvestphysiologicaldisordersincitrusfruitanditshormonalregulation[j].plsharvestbiotec,2008,48(1):146-149.？
|
| [20] | wanl,zhaw,chengx,etal.ariceβ？1,3？glucanasegeneosg1isrequiredforcallosedegradationinpollendevelopment[j].planta,2011,233(2):309-323.？
|
| [21] | 闫洁，陈守才，夏志辉.橡胶树死皮病胶乳c？乳清差异表达蛋白质的筛选与鉴定[j].中国生物工程杂志,2008,28(6):28-36.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
