以青钱柳二根一干苗为研究对象,采用裂区设计研究了3种光照条件(全光照、50%全光照、15%全光照)和3个种源(五峰、沅陵、沐川)对青钱柳叶中总三萜、阿江榄仁酸和青钱柳甙Ⅰ含量的影响,探讨了青钱柳叶中三萜类化合物含量的季节性动态变化。结果表明:以10月份作为青钱柳叶收获季节,不同处理青钱柳叶中三萜类化合物含量及产量存在显著差异。总三萜含量以沅陵种源+全光照处理最高,为42.03 mg/g; 阿江榄仁酸含量以沐川种源+15%全光照处理最高,为4.42 mg/g; 而青钱柳甙Ⅰ含量则以沐川种源+全光照处理最高,为2.52 mg/g。总三萜、青钱柳甙Ⅰ产量均以沐川种源+全光照处理最高,分别为2 907.2 mg/株、264.4 mg/株; 阿江榄仁酸产量以沅陵种源+50%全光照处理最高,为208.6 mg/株。三萜类化合物含量的季节动态显示,其最高值均出现在9月。青钱柳叶三萜类化合物含量与种源、光照强度以及叶片采摘时间密切相关,研究结果为青钱柳药用林的定向培育提供了支持。Based on the seedlings of Cyclocarya paliurus(1-year stem with 2 year root system), split-split plot design with three C. paliurus provenances(Wufeng, Yuanling and Muchuan)and three light conditions(full sunlight, 50% full sunlight and 15% full sunlight)was adopted to compare the content variation of total triterpenoid, arjunolic acid and cyclocarioside I in the leaves of C. paliurus seedlings under different treatments. Meanwhile, the seasonal variation of triterpenoid contents in the leaves was also discussed. The results showed that there were significant differences in triterpenoid accumulation in leaves of C. paliurus seedlings among different treatments when October is defined as leaf harvest time. The highest content of total triterpenoids(42.03 mg/g)was obtained in the treatment of Yuanling provenance with full sunlight, while the greatest contents of arjunolic acid and cyclocarioside I were achieved under the treatments of Muchuan provenance with 15% of full sunlight and Muchuan provenance with full sunlight, reaching 4.42 mg/g and 2.52 mg/g, respectively. The greatest production of total triterpenoids and cyclocarioside I was obtained in the treatment of Muchuan provenance with full sunlight, reaching 2 907.2 and 264.4 mg/plant respectively. However, the highest production of arjunolic acid was observed in the treatments of Yuanling provenance with 50% of full sunlight, hitting to 208.6 mg/plant. The accumulation of triterpenoids in the leaves of Cyclocarya paliurus are closely related to provenance, illumination intensity and the harvest time of leaves. Results from this study would provide some important practical and theoretical bases for effectively managing the plantations of C. paliurus
