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OALib Journal期刊
ISSN: 2333-9721
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枣实蝇及其他5种检疫性实蝇的pcr-rflp快速鉴定
DOI: 10.3969/j.issn.1000-2006.2014.01.033, PP. 183-186
Keywords: 枣实蝇,检疫性实蝇,pcr-rflp,线粒体dna
Abstract:
枣实蝇(carpomyavesuvianacosta)是一种新入侵检疫性有害生物。应用pcr-rflp技术,快速区分鉴定了枣实蝇与我国口岸截获频率较高的5种检疫性实蝇。研究表明:设计出的2对引物对6种供试实蝇线粒体dna(mtdna)进行pcr扩增,其扩增片断大小分别为350bp和450bp。pcr扩增产物用2种限制性内切酶drai和msei进行酶切,根据不同的酶切位点准确区分了6种供试的实蝇。此方法可用于口岸截获实蝇的快速检疫鉴定。
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