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杉木地理种源的est-ssr分子标记变异研究

DOI: 10.3969/j.issn.1000-2006.2014.01.001, PP. 1-8

Keywords: 杉木种源,ssr,地理等位变异,进化

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Abstract:

研究采用est-ssr分子标记对全国不同杉木种源区的42个代表性种源进行了地理种源分子标记的遗传多样性研究。实验结果表明,杉木种源水平上存在较高的遗传多态性。实验使用15个est-ssr引物扩增出17个位点,共有52个多态性等位基因,每个位点平均具3.0588个等位基因,平均每个座位的pic为0.296。相对其他研究,该研究利用ssr标记在每位点检测到更多的遗传变异信息。以遗传距离10作为阀值时,42个杉木种源聚类分析可知,除了福建永春碧卿(编号2)和广西浦北(编号16)种源自成一类外,其他40个种源按从南到北、从东到西的地理分布被聚为四大类群,与以往的杉木种子区划研究结果有较高的吻合度。同时,研究也检测到ssr分子标记的等位基因频率在四大类群间存在着较大的变异,而且这些标记大多位于与植物抗逆性相关的功能基因的编码区域内。因此,笔者推测杉木的地理变异可能与这些基因的等位变异有关。

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