|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
aflp指纹图谱技术在动物物种鉴定中的应用
DOI: 10.3969/j.jssn.1000-2006.2008.02.032, PP. 142-144
Keywords: 扩增片段长度多态性,动物物种鉴定,类羊,家牛,黑熊
Abstract:
用aflp(扩增片段长度多态性)方法来构建类牛、家牛和黑熊的dna指纹图谱，并结合国内实验室的具体情况，避免使用同位素;采用3条技术路线，并灵活应用了两种酶，同时切割和分别在连接寡聚核苦酸接头前后进行切割;在引物设计时应用3’端有差异的选择性引物进行pcr扩增，同时对实验结果进行比较。结果表明:两种酶分别在连接寡聚核苦酸接头前后进行切割，然后用单种引物扩增前一种酶切割的片断能获得比较好的结果。因此，aflp可用于野生动物dna分子水平上的鉴定。
| [1] | [1jpieterv,reneh.aflp:anewtechniquefordnafingerprinting[jj.nucleicacidsres,1995,21:4407-4414.
|
| [2] | [jj.molecularphylogeneticsandevolution,2004,31(3):910-914.
|
| [3] | [5jkalitam,malekw.applicationoftheaflpmethodtodifferentiategenistαtinctoriαmicrosymbionts[jj.jgenapplmicrobiol,2006,52(6):
|
| [4] | 111(pt2):213-223.
|
| [5] | [7jurbanellis,dellarosev.dna-fingerprinting(aflpandrflp)forgenotypicidentificationinspeciesofthepleurotuseryngiicomplex[jj.
|
| [6] | [9jvelappann,sondgrassjl.rapididentificationofpathogenicbacteriabysingleenzymeamplifiedfragmentlengthpolymorphismanalysis[jj.
|
| [7] | diagnosticmicrobiologyandinfectionsdisease,2001,39:77-83.
|
| [8] | [2jgaroiaf,guarnieroi.comparativeanalysisofaflpsandssrsefficiencyinresolvingpopulationgeneticstructureofmediterraneansoleαvulgαris
|
| [9] | [jj.molecol,2007,16(7):1377-1387.
|
| [10] | [3j苟本富，邹国林.分子标记技术及其应用研究进展[jj.渝西学院学报，2002，15(1):22-30.
|
| [11] | [4jalexandreriberon,claudemiaud.geneticvariationinanendemicsalamander,sαlαmαndraαtra，usingamplifiedfragmentlengthpolymorphism
|
| [12] | 321-328.
|
| [13] | [6jsereniusm,man旧neno.geneticdifferentiationinpyrenophoraterespopulationsmeasuredwithaflpmarkers[jj.mycolres,2007,
|
| [14] | applmicrobiolbiotechnol,2007,74(3):592-600.
|
| [15] | [8jjmoneam,aimonemarsonp.aflptmmarkerfordnafingerprintingincattle[jj.animalgenetics,1997,28:418-426.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
