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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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火炬松体胚发生与几种专利培养基的应用
DOI: 10.3969/j.jssn.1000-2006.2008.01.001
Keywords: 火炬松,体细胞胚胎发生,专利培养基,诱导率,胚性胚柄细胞团
Abstract:
以火炬松优良家系20692、20615、201036、20639不同球果采收期的含未成熟合子胚的雌配子体为外植体,利用4种针叶树体胚发生专利培养基al、tx、lp、ds,分别与al、tx、lp培养基激素组合及无激素组合形成16种诱导培养基,开展不同家系、基本培养基、球果采收期对火炬松体细胞胚胎发生率影响的研究。结果表明:合子胚发育的第2、3、4阶段为最理想外植体，不同家系、基本培养基、激素组合对esm诱导率及外植体褐化率均存在显著影响，16种组合的培养基中,在lplp、lptx、altx培养基上可获得较高的体胚诱导率,分别为18%、16%、11%.
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