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OALib Journal期刊
ISSN: 2333-9721
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青钱柳愈伤组织不定芽诱导技术
DOI: 10.3969/j.issn.1000-2006.2014.02.011, PP. 52-56
Keywords: 青钱柳,外植体,激素,愈伤组织,不定芽
Abstract:
以青钱柳成熟种子培养无菌苗,再用无菌苗叶和茎段为外植体进行组织培养,观察了愈伤组织形成及再分化形成不定芽的过程,分析了不同培养基和生长调节物质对青钱柳愈伤组织及不定芽发生的影响。结果表明:(1)ms、wpm和b5培养基均能诱导出愈伤组织,茎段和叶片的诱导率最高分别达到90.00%和86.70%;(2)激素及其浓度对愈伤组织诱导均有显著影响。当加入tdz质量浓度为0.5mg/l时,叶愈伤组织诱导率达95.00%;而加入tdz质量浓度为0.1mg/l时,茎段诱导率达92.85%;(3)叶愈伤组织在wpm+1.0mg/l6-ba+0.1mg/liba的培养基上,不定芽的诱导频率最高,达53.85%,这实现了青钱柳由愈伤组织分化形成不定芽和萌发增殖的过程,但萌发率较低。
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