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OALib Journal期刊
ISSN: 2333-9721
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转基因小黑杨对土壤微生物群落结构的影响
DOI: 10.3969/j.issn.1000-2006.2014.02.015, PP. 75-80
Keywords: 转基因小黑杨,土壤微生物,基因水平转移
Abstract:
为评价转基因林木环境释放可能引起的生态风险,以及转基因林木在生产应用中的可行性和可靠性,以实验室种植的转bt-蜘蛛神经毒肽重组基因的小黑杨为材料,对转基因与非转基因植株根际(通常为几毫米至几厘米)土壤细菌、放线菌、真菌数量和基因水平转移情况进行检测,结果表明:转基因小黑杨种植一段时间后其根系附近的微生物数量略高于非转基因小黑杨。利用卡那霉素选择培养基分离根系周围细菌菌落发现转基因植株周围的土样细菌中,kanr抗性细菌菌落数占总细菌菌落的比例在11.58%～13.00%之间,而非转基因植株根际周围和空白土壤细菌菌落数则在5.73%～6.40%之间,表明转基因小黑杨根系对土壤细菌的抗生素抗性产生一定的影响。利用转基因植株的目的基因和抗性基因设计引物,以土壤中细菌基因组dna为模板进行pcr扩增,转基因植株种植7个月后,根际土壤细菌菌落中含有抗性基因的菌落数量明显增加。虽然在种植1个月的转基因植株根际土壤土样细菌中未检出目的基因,但在种植7个月后的土样中检验到了很少的目的基因阳性细菌,阳性细菌菌落比例为2%～5%,检测结果表明可能有极少数bt-蜘蛛神经毒肽重组基因发生了水平转移。
| [1] | wolfenbargerll,phiferpr.theecologicalrisksandbenefitsofgeneticallyengineeredplants[j].science,2000,290:2088-2093.
|
| [2] | 马骏,高必达,万方浩,等.转bt基因抗虫棉的生态风险及治理对策[j].应用生态学报,2003,14(3):443-446.maj,gaobd,wanfh,etal.ecologicalriskofbttransgeniccottonanditsmanagementstrategy[j].chinesejournalofappliedecology,2003,14(3):443-446.
|
| [3] | 胡建军,杨敏生,卢孟柱.我国抗虫转基因杨树生态安全性研究进展[j].生物多样性,2010,18(4):336-345.hujj,yangms,lumz.advancesinbiosafetystudiesontransgenicinsect-resistantpoplars[j].biodiversityscience,2010,18(4):336-345.
|
| [4] | castaldinim,turrinia,sbranac,etal.impactofbtcornonrhizosphericandsoileubacterialcommunitiesandonbeneficialmycorrhizalsymbiosisinexperimentalmicrocosms[j].appliedandenvironmentalmicrobiology,2005,71(11):6719-6729.
|
| [5] | 甄志先,王进茂,杨敏生.转抗虫基因杨树对土壤微生物影响分析[j].河北农业大学学报,2011,34(1):78-81.zhenzx,wangjm,yangms.effectsoftransgenicinsect-resistancehybridpoplar741grovesonsoilmicroorganisms[j].journalofagriculturaluniversityofhebei,2011,34(1):78-81.
|
| [6] | 崔金杰,雒？瑜,李树红,等.转基因抗虫棉对土壤微生物影响的初步研究[j].河北农业大学学报,2005,28(6):73-75.cuijj,luojy,lish,etal.preliminarystudyintheeffectsoftransgeniccottonfieldsonthesoilmicrobe[j].journalofagriculturaluniversityofhebei,2005,28(6):73-75.
|
| [7] | 刘岩.转抗虫基因小黑杨根系分泌物对土壤生态系统的影响[d].哈尔滨:东北林业大学,2009.liuy.influenceoftransgenicpopulussimonii×p.nigra'srootsecretiononsoilecosystem[d].harbin:northeastforestryuniversity,2009.
|
| [8] | 许光辉,郑洪元.土壤微生物分析方法手册[m].上海:上海科学技术出版社,1986.
|
| [9] | 向万胜,吴金水,肖和艾,等.土壤微生物的分离、提取与纯化研究进展[j].应用生态学报,2003,14(3):453-456.xiangws,wujs,xiaoha,etal.advancesinextractionandpurificationofsoilmicroorganism[j].chinesejournalofappliedecology,2003,14(3):453-456.
|
| [10] | hilbecka,baumgartnerm,friedpm,etal.effectsoftransgenicbacillusthuringiensiscorn-fedpreyonmortalityanddevelopmenttimeofimmaturechrysoperlacornea(neuroptera:chrysopidae)[j].environmentalentomology,1998,27(2):480-487.
|
| [11] | 俞元春,冷春龙,舒洪岚,等.转基因抗虫棉对土壤养分和酶活性的影响[j].南京林业大学学报:自然科学版,2011,35(5):21-24.shuyc,lengcl,shuhl,etal.effectsofinsect-resistanttransgeniccottononsoilnutrientsandenzymeactivities[j].journalofnanjingforestryuniversity:naturalsciencesedition,2011,35(5):21-24.
|
| [12] | searsmk,hellmichrl,stanley-hornde,etal.impactofbtcornpollenonmonarchbutterflypopulations:ariskassessment[j].proceedingsofthenationalacademyofsciences,2001,98(21):11937-11942.
|
| [13] | 张雁,郭同斌,潘惠新,等.转bt基因南林895杨抗虫性及对土壤微生物影响分析[j].林业科学研究,2012,25(3):346-350.zhangy,guotb,panhx,etal.analysisoninsecticidalactivityofbttransgenicpopulusdeltoides×p.euramericanacv.‘nanlin895'anditseffectsonsoilmicroorganism[j].forestresearch,2012,25(3):346-350.
|
| [14] | axelssonep,hjälténj,leroycj,etal.leaflitterfrominsect-resistanttransgenictreescauseschangesinaquaticinsectcommunitycomposition[j].journalofappliedecology,2011,48(6):1472-1479.
|
| [15] | 徐广惠,王宏燕,刘佳.抗草甘膦转基因大豆(rrs)对根际土壤细菌数量和多样性的影响[j].生态学报,2009,29(8):4535-4541xugh,wanghy,liuj.effectsofrrsontheamountanddiversityofbacteriainrhizosphericsoil[j].actaecologicasinica,2009,29(8):4535-4541.
|
| [16] | daniellh.molecularstrategiesforgenecontainmentintransgeniccrops[j].naturebiotechnology,2002,20(6):581-586.
|
| [17] | 卢宝荣,张文驹,李博.转基因的逃逸及生态风险[j].应用生态学报,2003,14(6):989-994.lubr,zhangwj,lib.escapeoftransgenesanditsecologicalrisks[j].chinesejournalofappliedecology,2003,14(6):989-994.
|
| [18] | 钱迎倩,魏伟,桑卫国,等.转基因作物对生物多样性的影响[j].生态学报,2001,21(3):337-343.qianyq,weiw,sangwg,etal.effectsoftransgeniccropsonbiodiversity[j].actaecologicasinica,2001,21(3):337-343
|
| [19] | 康向阳,刘志民,李胜功.论转基因林木的潜在生态风险性[j].应用生态学报,2004,15(7):1281-1284.kangxy,liuzm,lisg.potentialecologicalrisksoftransgenictrees[j].chinesejournalofappliedecology,2004,15(7):1281-1284.
|
| [20] | wuwx,yeqf,mah,etal.bt-transgenicricestrawaffectstheculturablemicrobiotaanddehydrogenaseandphosphataseactivitiesinafloodedpaddysoil[j].soilbiologyandbiochemistry,2004,36(2):289-295.
|
| [21] | 娜日苏,红雨,杨殿林,等.黄河流域棉区转bt基因棉种植对根际土壤微生物数量及细菌多样性的影响[j].应用生态学报,2011,22(1):114-120.nars,hongy,yangdl,etal.effectofplantationoftransgenicbtcottonontheamountofrhizospheresoilmicroorganismandbacterialdiversityinthecottonregionofyellowriverbasin[j].chinesejournalofappliedecology,2011,22(1):114-120.
|
| [22] | 顾立江,李燕玲,杜克久.转基因毛白杨外源基因转移的环境风险分析[j].环境化学,2009,28(6):838-841.gulj,liyl,dukj.theenvironmentalriskassayofaforeigngenetransferfromtransgenicpopulustomentosacarr.tomicroorganisms[j].environmentalchemistry,2009,28(6):838-841.
|
| [23] | 方生佐,徐锡增,严相进,等.修枝强度和季节对杨树人工林生长的影响[j].南京林业大学学报,2000,24(6):6-10.fangsz,xuxz,yanxj,etal.effectsofpruningintensitiesandpruningseasonsonthegrowthdynamicsofthepoplarplantation[j].journalofnanjingforestryuniversity,2000,24(6):6-10.
|
| [24] | 赵勇,周志华,李武,等.土壤微生物分子生态学研究中总dna的提取[j].农业环境科学学报,2005,24(5):854-860.zhaoy,zhouzh,liw,etal.dnaextractionfromsoilformoecularmicrobialcommunityanalysis[j].journalofagro-environmentscience,2005,24(5):854-860.
|
| [25] | 贺林.解码生命[m].北京:科学出版社,2000.
|
| [26] | 陈宏宇,李晓鸣,王敬国.抗病性不同大豆品种根面及根际微生物区系的变化ⅰ.非连作大豆(正茬)根面及根际微生物区系的变化[j].植物营养与肥料学报,2005,11(6):804-809.chenhy,lixm,wangjg.changeofmicrofloraintherhizoplaneandrhizosphereofdifferentdiseaseresistancesoybeancultivarsⅰ.changeofmicrofloraintherhizoplaneandrhizosphereofsoybeanundernormalrotationcrop[j].plantnutritionandfertilizerscience,2005,11(6):804-809.
|
| [27] | 王忠华,叶庆富,舒庆尧,等.转基因植物根系分泌物对土壤微生态的影响[j].应用生态学报,2002,13(3):373-375.wangzh,yeqf,shuqy,etal.impactofrootexudatesfromtransgenicplantsonsoilmicro-ecosystems[j].chinesejournalofappliedecology,2002,13(3):373-375.
|
| [28] | ogerp,petita,dessauxy.geneticallyengineeredplantsproducingopinesaltertheirbiologicalenvironment[j].naturebiotech,1997,15(4):369-372
|
| [29] | donegankk,schallerdl,stonejk,etal.microbialpopulations,fungalspeciesdiversityandplantpathogenlevelsinfieldplotsofpotatoplantsexpressingthebacillusthuringiensisvar.tenebrionisendotoxin[j].transgenicresearch,1996,5(1):25-35.
|
| [30] | glandorfdcm,barkerp,vanloonlc.influenceoftheproductionofantibacterialandantifungalproteinsbytransgenicplantsonthesaprophyticsoilmicroflora[j].actabotneerl,1997,46(1):85-104.
|
| [31] | hoffmannt,golzc,schiedero.foreigndnasequencesarereceivedbyawild-typestrainofaspergillusnigerafterco-culturewithtransgenichigherplants[j].currentgenetics,1994,27(1):70-76.
|
| [32] | moonsukhur,youngwoonlim,jaejeongyu,etal.fungalcommunityassociatedwithgeneticallymodifiedpoplarduringmetalphytoremediation[j].thejournalofmicrobiology,2012,50(6):910-915.
|
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